Department of Micro, and Nanotechnology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs Lyngby, Denmark.
Department of Micro, and Nanotechnology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs Lyngby, Denmark.
Talanta. 2018 May 15;182:49-54. doi: 10.1016/j.talanta.2018.01.056. Epub 2018 Feb 20.
In this study, we developed a new type of multifunctional molecularly imprinted polymer (MIP) composite as an all-in-one biosensor for the low-cost, rapid and sensitive detection of doxycycline in pig plasma. The MIP composite consisted of a magnetic core for ease of manipulation, and a shell of fluorescent MIPs for selective recognition of doxycycline. By simply incorporating a small amount of fluorescent monomer (fluorescein-O-acrylate), the fluorescent MIP layer was successfully grafted onto the magnetic core via a surface imprinting technique. The resultant MIP composites showed significant doxycycline-dependent fluorescence quenching in an aqueous environment. Good linearity ranging from 0.2 to 6 µM was achieved, and the limit of detection was determined to be 117 nM. The biosensor also showed good selectivity towards doxycycline when compared to other common antibiotic residues. The multifunctional MIP composites were used to directly extract doxycycline from spiked pig plasma samples and quantify the antibiotics based on the quenched fluorescence signals. Recoveries of doxycycline were found in the range of 88-107%.
在本研究中，我们开发了一种新型的多功能分子印迹聚合物（MIP）复合材料，作为一种一体化的生物传感器，用于低成本、快速和灵敏地检测猪血浆中的强力霉素。该 MIP 复合材料由一个磁性核组成，便于操作，还有一层荧光 MIP 作为对强力霉素的选择性识别。通过简单地加入少量荧光单体（荧光素-O-丙烯酰胺），荧光 MIP 层通过表面印迹技术成功地接枝到磁性核上。所得 MIP 复合材料在水相中表现出显著的强力霉素依赖性荧光猝灭。在 0.2 至 6 µM 的范围内实现了良好的线性关系，检测限确定为 117 nM。与其他常见的抗生素残留相比，该生物传感器对强力霉素也表现出良好的选择性。多功能 MIP 复合材料用于直接从加标猪血浆样品中提取强力霉素，并根据猝灭的荧光信号定量分析抗生素。强力霉素的回收率在 88-107%范围内。
