iMSD 和 2D-pCF 分析在体内细胞分子运动研究中的比较：以表皮生长因子受体为例。
Comparison between iMSD and 2D-pCF analysis for molecular motion studies on in vivo cells: The case of the epidermal growth factor receptor.
机构信息
Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, USA; Área de Investigación Respiratoria, Departamento de Fisiopatología, Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Uruguay.
Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, USA; Dipartimento di Fisica e Chimica, Università di Palermo, Italy.
出版信息
Methods. 2018 May 1;140-141:74-84. doi: 10.1016/j.ymeth.2018.01.010. Epub 2018 Mar 1.
Abstract
Image correlation analysis has evolved to become a valuable method of analysis of the diffusional motion of molecules in every points of a live cell. Here we compare the iMSD and the 2D-pCF approaches that provide complementary information. The iMSD method provides the law of diffusion and it requires spatial averaging over a small region of the cell. The 2D-pCF does not require spatial averaging and it gives information about obstacles for diffusion at pixel resolution. We show the analysis of the same set of data by the two methods to emphasize that both methods could be needed to have a comprehensive understanding of the molecular diffusional flow in a live cell.
摘要
图像相关分析已经发展成为分析活细胞中分子扩散运动的一种有价值的方法。在这里，我们比较了 iMSD 和 2D-pCF 方法，它们提供了互补的信息。iMSD 方法提供了扩散定律，它需要对细胞的小区域进行空间平均。2D-pCF 不需要空间平均，它以像素分辨率给出了扩散障碍的信息。我们通过两种方法对同一组数据进行分析，以强调这两种方法都可能需要对活细胞中的分子扩散流有一个全面的了解。
相似文献
1
Comparison between iMSD and 2D-pCF analysis for molecular motion studies on in vivo cells: The case of the epidermal growth factor receptor.iMSD 和 2D-pCF 分析在体内细胞分子运动研究中的比较：以表皮生长因子受体为例。
Methods. 2018 May 1;140-141:74-84. doi: 10.1016/j.ymeth.2018.01.010. Epub 2018 Mar 1.
2
Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells.活细胞细胞膜上肌动蛋白丝结合蛋白 1 动力学的定量图像均方根位移（iMSD）分析。
Methods. 2018 May 1;140-141:119-125. doi: 10.1016/j.ymeth.2017.12.002. Epub 2017 Dec 11.
3
Diffusion Tensor Analysis by Two-Dimensional Pair Correlation of Fluorescence Fluctuations in Cells.通过细胞中荧光涨落的二维对关联进行扩散张量分析。
Biophys J. 2016 Aug 23;111(4):841-851. doi: 10.1016/j.bpj.2016.07.005.
4
Circle scanning STED fluorescence correlation spectroscopy to quantify membrane dynamics and compartmentalization.环形扫描 STED 荧光相关光谱法定量测量膜动力学和区隔化。
Methods. 2018 May 1;140-141:188-197. doi: 10.1016/j.ymeth.2017.12.005. Epub 2017 Dec 16.
5
A straightforward STED-background corrected fitting model for unbiased STED-FCS analyses.一种用于无偏 STED-FCS 分析的直截了当的 STED 背景校正拟合模型。
Methods. 2018 May 1;140-141:212-222. doi: 10.1016/j.ymeth.2018.02.010. Epub 2018 Feb 14.
6
Velocity landscape correlation resolves multiple flowing protein populations from fluorescence image time series.速度景观关联从荧光图像时间序列中解析出多个流动蛋白质群体。
Methods. 2018 May 1;140-141:126-139. doi: 10.1016/j.ymeth.2018.02.011. Epub 2018 Feb 16.
7
Two-detector number and brightness analysis reveals spatio-temporal oligomerization of proteins in living cells.双探测器计数和亮度分析揭示活细胞中蛋白质的时空寡聚化。
Methods. 2018 May 1;140-141:161-171. doi: 10.1016/j.ymeth.2018.03.007. Epub 2018 Mar 21.
8
Accuracy and dynamic range of spatial image correlation and cross-correlation spectroscopy.空间图像相关和互相关光谱学的准确性与动态范围。
Biophys J. 2005 Aug;89(2):1251-60. doi: 10.1529/biophysj.104.057364. Epub 2005 May 27.
9
Image mean square displacement to study the lateral mobility of Angiotensin II type 1 and Endothelin 1 type A receptors on living cells.利用图像均方位移研究活细胞上血管紧张素 II 型 1 受体和内皮素 1 型 A 受体的侧向流动性。
Microsc Res Tech. 2020 Apr;83(4):381-392. doi: 10.1002/jemt.23425. Epub 2019 Dec 13.
10
Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.利用激光扫描显微镜的涨落关联光谱法：探索隐藏的时间结构
Biophys J. 2005 May;88(5):L33-6. doi: 10.1529/biophysj.105.061788. Epub 2005 Mar 25.
引用本文的文献
1
The Heterogeneous Diffusion of Polystyrene Nanoparticles and the Effect on the Expression of Quorum-Sensing Genes and EPS Production as a Function of Particle Charge and Biofilm Age.聚苯乙烯纳米颗粒的异质扩散及其对群体感应基因表达和胞外聚合物产生的影响与颗粒电荷和生物膜年龄的关系
Environ Sci Nano. 2023 Sep 1;10(9):2551-2565. doi: 10.1039/d3en00219e. Epub 2023 Aug 18.
2
Barriers to Diffusion in Cells: Visualization of Membraneless Particles in the Nucleus.细胞中扩散的障碍：细胞核中无膜颗粒的可视化
Biophysicist (Rockv). 2020 Aug;1(2). doi: 10.35459/tbp.2019.000111. Epub 2020 Aug 13.
3
Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry.荧光波动光谱技术可定量研究钾离子通道亚基动力学和化学计量。
Sci Rep. 2021 May 21;11(1):10719. doi: 10.1038/s41598-021-90002-2.
4
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector.使用蔡司 Airyscan 检测器对细胞区室进行超分辨率动态指纹图谱的综合相关分析。
Nat Commun. 2018 Nov 30;9(1):5120. doi: 10.1038/s41467-018-07513-2.
本文引用的文献
1
Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions.通过二维空间对交叉相关可视化活细胞中分子扩散的屏障和障碍。
Biomed Opt Express. 2017 Dec 20;9(1):303-321. doi: 10.1364/BOE.9.000303. eCollection 2018 Jan 1.
2
Diffusion Tensor Analysis by Two-Dimensional Pair Correlation of Fluorescence Fluctuations in Cells.通过细胞中荧光涨落的二维对关联进行扩散张量分析。
Biophys J. 2016 Aug 23;111(4):841-851. doi: 10.1016/j.bpj.2016.07.005.
3
Fast spatiotemporal correlation spectroscopy to determine protein lateral diffusion laws in live cell membranes.快速时空相关光谱法测定活细胞膜中蛋白质的侧向扩散规律。
Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12307-12. doi: 10.1073/pnas.1222097110. Epub 2013 Jul 8.
4
Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medium.考虑定位误差的单粒子轨迹的均方位移分析：各向同性介质中的布朗运动。
Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Oct;82(4 Pt 1):041914. doi: 10.1103/PhysRevE.82.041914. Epub 2010 Oct 20.
5
Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments.单平面照明显微荧光相关光谱技术（SPIM-FCS）可探测非均匀三维环境。
Opt Express. 2010 May 10;18(10):10627-41. doi: 10.1364/OE.18.010627.
6
Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopy.通过成像荧光相关光谱技术研究扩散、传输和细胞膜组织。
Biophys J. 2009 Nov 4;97(9):2630-9. doi: 10.1016/j.bpj.2009.08.025.
7
Imaging barriers to diffusion by pair correlation functions.通过对关联函数研究扩散的成像障碍。
Biophys J. 2009 Jul 22;97(2):665-73. doi: 10.1016/j.bpj.2009.04.048.
8
k-Space image correlation spectroscopy: a method for accurate transport measurements independent of fluorophore photophysics.k空间图像相关光谱法：一种独立于荧光团光物理性质的精确传输测量方法。
Biophys J. 2006 Oct 15;91(8):3061-75. doi: 10.1529/biophysj.106.082768. Epub 2006 Jul 21.
9
Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells.时空图像相关光谱学（STICS）理论、验证及其在活CHO细胞中蛋白质速度映射的应用。
Biophys J. 2005 May;88(5):3601-14. doi: 10.1529/biophysj.104.054874. Epub 2005 Feb 18.
10
gamma-glutamyl leukotrienase, a gamma-glutamyl transpeptidase gene family member, is expressed primarily in spleen.γ-谷氨酰白三烯酶，一种γ-谷氨酰转肽酶基因家族成员，主要在脾脏中表达。
J Biol Chem. 1998 Oct 23;273(43):28277-85. doi: 10.1074/jbc.273.43.28277.
Zotero 插件