Department of Neurology, Washington University School of Medicine, St. Louis, MO, USA; Knight Alzheimer's Disease Research Center, Washington University School of Medicine, St. Louis, MO, USA; Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MO, USA.
Department of Neurology, Washington University School of Medicine, St. Louis, MO, USA; Knight Alzheimer's Disease Research Center, Washington University School of Medicine, St. Louis, MO, USA; Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO, USA.
Alzheimers Dement. 2018 Nov;14(11):1460-1469. doi: 10.1016/j.jalz.2018.01.013. Epub 2018 Mar 2.
Levels of amyloid β peptide 42 (Aβ42), total tau, and phosphorylated tau-181 are well-established cerebrospinal fluid (CSF) biomarkers of Alzheimer's disease, but variability in manual plate-based assays has limited their use. We examined the relationship between CSF biomarkers, as measured by a novel automated immunoassay platform, and amyloid positron emission tomography.
CSF samples from 200 individuals underwent separate analysis for Aβ42, total tau, and phosphorylated tau-181 with automated Roche Elecsys assays. Aβ40 was measured with a commercial plate-based assay. Positron emission tomography with Pittsburgh Compound B was performed less than 1 year from CSF collection.
Ratios of CSF biomarkers (total tau/Aβ42, phosphorylated tau-181/Aβ42, and Aβ42/Aβ40) best discriminated Pittsburgh Compound B-positive from Pittsburgh Compound B-negative individuals.
CSF biomarkers and amyloid positron emission tomography reflect different aspects of Alzheimer's disease brain pathology, and therefore, less-than-perfect correspondence is expected. Automated assays are likely to increase the utility of CSF biomarkers.
β淀粉样蛋白 42（Aβ42）、总 tau 和磷酸化 tau-181 的水平是阿尔茨海默病的既定脑脊液（CSF）生物标志物，但其基于平板的手动检测方法的变异性限制了其应用。我们研究了通过新型自动化免疫测定平台测量的 CSF 生物标志物与淀粉样蛋白正电子发射断层扫描之间的关系。
对 200 名个体的 CSF 样本进行了单独分析，使用自动化罗氏 Elecsys 检测方法分别检测 Aβ42、总 tau 和磷酸化 tau-181。使用商业平板检测方法检测 Aβ40。在 CSF 采集后不到 1 年进行了使用匹兹堡化合物 B 的正电子发射断层扫描。
CSF 生物标志物（总 tau/Aβ42、磷酸化 tau-181/Aβ42 和 Aβ42/Aβ40）比值可最佳区分匹兹堡化合物 B 阳性和匹兹堡化合物 B 阴性个体。
CSF 生物标志物和淀粉样蛋白正电子发射断层扫描反映了阿尔茨海默病大脑病理学的不同方面，因此预计会存在不太一致的情况。自动化检测方法可能会增加 CSF 生物标志物的实用性。
