The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, P. R. China.
Key Laboratory for Animal-derived Food Safety of Henan Province, Zhengzhou, Henan, P. R. China.
Nanomedicine. 2018 Jun;14(4):1201-1212. doi: 10.1016/j.nano.2018.02.010. Epub 2018 Mar 1.
Inactivated transmissible gastroenteritis virus (TGEV) vaccines are widely used in swine herds in China. These are limited, however, by the need to elicit both humoral and cellular immunity, as well as the efficiency of adjuvants. In this study, a 70-nm nano silicon particle was applied with inactivated TGEV vaccine in mice, and its immune-enhancing effects and mechanism of action investigated. We found that nano silicon applied with inactivated TGEV vaccine induced high antibody titers, increase IL-6, TNF-α and IFN-γ expression, and stimulate CD3+ T cell proliferation with a high CD4+/CD8+ T lymphocyte ratio. Nano silicon could quickly activate innate and adaptive immunity by stimulating Toll-like receptor signaling pathways, indicating that the nano silicon adjuvant enhanced long-term humoral and early cellular immune responses when combined with inactivated TGEV vaccine. Nano silicon could be considered for use as an antigen- carrier and adjuvant for veterinary vaccines.
在中国，猪群中广泛使用灭活传染性胃肠炎病毒 (TGEV) 疫苗。然而，这些疫苗受到需要引发体液和细胞免疫以及佐剂效率的限制。在这项研究中，将 70nm 纳米硅颗粒与灭活 TGEV 疫苗一起应用于小鼠，并研究其免疫增强作用及其作用机制。我们发现，纳米硅与灭活 TGEV 疫苗联合使用可诱导高抗体滴度，增加 IL-6、TNF-α 和 IFN-γ 的表达，并刺激 CD3+T 细胞增殖，具有较高的 CD4+/CD8+T 淋巴细胞比值。纳米硅通过刺激 Toll 样受体信号通路可迅速激活先天和适应性免疫，表明纳米硅佐剂与灭活 TGEV 疫苗联合使用时可增强长期体液和早期细胞免疫反应。纳米硅可用作兽医疫苗的抗原载体和佐剂。
