Pei Wenhua, Lu Tengfei, Wang Kunfu, Ji Meng, Zhang Shuang, Chen Fenghao, Li Lu, Li Xiangchen, Guan Weijun
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanmingyuan West road, haidian district, Beijing, 100193, China.
College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China.
Cytotechnology. 2018 Jun;70(3):1009-1021. doi: 10.1007/s10616-017-0115-2. Epub 2018 Mar 3.
Mesenchymal stem cells derived from amniotic fluid have become one of the most potential stem cell source for cell-based therapy for the reason they can be harvested at low cost and without ethical problems. Here, we obtained amniotic fluid stem cells (AFSCs) from ovine amniotic fluid and studied the expansion capacity, cell markers expression, karyotype, and multilineage differentiation ability. In our work, AFSCs were subcultured to passage 62. The cell markers, CD29, CD44, CD73 and OCT4 which analyzed by RT-PCR were positive; CD44, CD73, CD90, CD105, NANOG, OCT4 analyzed by immunofluorescence and flow cytometry were also positive. The growth curves of different passages were all typically sigmoidal. The different passages cells took on a normal karyotype. In addition, AFSCs were successfully induced to differentiate into adipocytes, osteoblasts and chondrocytes. The results suggested that the AFSCs isolated from ovine maintained normal biological characteristics and their multilineage differentiation potential provides many potential applications in cell-based therapies and tissue engineering.
羊水来源的间充质干细胞已成为基于细胞治疗中最具潜力的干细胞来源之一，因为它们可以低成本获取且不存在伦理问题。在此，我们从羊羊水获得羊水干细胞（AFSCs），并研究了其扩增能力、细胞标志物表达、核型及多向分化能力。在我们的研究中，AFSCs传代至62代。通过RT-PCR分析的细胞标志物CD29、CD44、CD73和OCT4呈阳性；通过免疫荧光和流式细胞术分析的CD44、CD73、CD90、CD105、NANOG、OCT4也呈阳性。不同传代的生长曲线均呈典型的S形。不同传代细胞具有正常核型。此外，AFSCs成功诱导分化为脂肪细胞、成骨细胞和软骨细胞。结果表明，从羊分离的AFSCs保持正常生物学特性，其多向分化潜能在基于细胞的治疗和组织工程中有许多潜在应用。
