Carneiro Joao A M, Canisso Igor F, Bandeira R S, Scheeren V F C, Freitas-Dell'Aqua Camila P, Alvarenga Marco A, Papa Frederico O, Dell'Aqua Jose A
Sao Paulo State University (UNESP), School of Veterinary Medicine and Animal Science, Botucatu, Brazil.
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL, 61802, USA.
Anim Reprod Sci. 2018 May;192:107-118. doi: 10.1016/j.anireprosci.2018.02.020. Epub 2018 Feb 24.
This study aimed to evaluate the antioxidant properties of coenzyme Q10 (CoQ10) during cryopreservation of semen obtained from stallions having good and bad semen freezing ability (GFA vs. BFA, respectively). Forty ejaculates (n = 20 stallions) were split into five centrifugation and five freezing extenders containing different concentrations of CoQ10 (0, 25, 50, 75 and 100 μmols/L). If CoQ10 was added to the centrifugation extender, the freezing extender had no CoQ10 added; similarly, if CoQ10 was added to the freezing extender, the centrifugation extender had no CoQ10. Semen cryopreserved on extenders containing no CoQ10 served as the control. After post-thaw total sperm motility (TM) assessments, the stallions were classified as GFA (i.e., decrease of ≤25% in TM, n = 7) or BFA (i.e., decrease of ≥40% in TM, n = 5). Stallions not fitting (n = 8) this enrollment criteria had samples discarded. After that, two straws for each extender were thawed at 37 °C for 30 s; one straw was immediately used for evaluation of sperm kinetics, plasma membrane integrity, non-capacitated spermatozoa, reactive oxygen species production, mitochondrial activity and lipid peroxidation. The second straw was kept at 37 °C for 30 min and subjected to the same assessments. Expectedly, sperm motility parameters were significantly lower for stallions with BFA. There were no effects of CoQ10 concentration or time for all parameters evaluated in the group with GFA when compared with the control extender (p > 0.05), except lipid peroxidation (p < 0.05). However, stallions with BFA had improved sperm parameters for samples processed with extenders containing CoQ10 (particularly 75 μmols/L) (p < 0.05), except for the reactive oxygen species production and mitochondrial potential (T0) in which there were no differences between the groups (p > 0.05). In summary, 75 μmols/L appears to be the optimal dose of Co-Q10, particularly, when added to the centrifugation extender.
本研究旨在评估辅酶Q10（CoQ10）在冷冻保存分别具有良好和不良精液冷冻能力（分别为GFA和BFA）的种马精液过程中的抗氧化特性。40份射精样本（n = 20匹种马）被分为五组，分别置于含有不同浓度CoQ10（0、25、50、75和100 μmol/L）的五种离心稀释液和五种冷冻稀释液中。如果CoQ10添加到离心稀释液中，冷冻稀释液则不添加CoQ10；同样，如果CoQ10添加到冷冻稀释液中，离心稀释液则不添加CoQ10。在不含CoQ10的稀释液中冷冻保存的精液作为对照。在解冻后评估总精子活力（TM）后，种马被分类为GFA（即TM降低≤25%，n = 7）或BFA（即TM降低≥40%，n = 5）。不符合该纳入标准的种马（n = 8）的样本被丢弃。之后，每种稀释液的两根细管在37°C解冻30秒；一根细管立即用于评估精子动力学、质膜完整性、未获能精子、活性氧产生、线粒体活性和脂质过氧化。第二根细管在37°C保存30分钟，并进行相同的评估。不出所料，BFA种马的精子活力参数显著较低。与对照稀释液相比，在GFA组中评估的所有参数，CoQ10浓度和时间均无影响（p > 0.05），脂质过氧化除外（p < 0.05）。然而，对于用含有CoQ10（特别是75 μmol/L）的稀释液处理的样本，BFA种马的精子参数有所改善（p < 0.05），活性氧产生和线粒体电位（T0）除外，各组之间无差异（p > 0.05）。总之，75 μmol/L似乎是Co-Q10的最佳剂量，特别是当添加到离心稀释液中时。
