Huang Danping, Li Hanmin, He Qidi, Yuan Weiqu, Chen Zuanguang, Yang Hongzhi
1Guangdong Key Laboratory of Liver Disease Research, The Third Affiliated Hospital, Sun Yat-sen University, Tianhe Road No. 600, Guangzhou, 510000 China.
2Hubei Provincial Hospital of Traditional Chinese Medicine, Garden Hill No. 4 Wuchang District, Wuhan, 430061 China.
Water Air Soil Pollut. 2018;229(3):81. doi: 10.1007/s11270-018-3739-8. Epub 2018 Feb 22.
Diethylnitrosamine (DEN) is present in food, water, and daily supplies and is regarded as a toxicant of carcinogenicity. The developmental toxicity of DEN has been rarely reported as yet. In this study, zebrafish were exposed to different concentrations of DEN at 6 h post-fertilization (hpf) to access embryonic toxicity of the compound. The results show that DEN resulted in negative effects of hatching rate, heartbeat, body length, and spontaneous movement. Deformities, including notochord malformation, pericardium edema, embryonic membrane turbidity, tail hypoplasia, yolk sac deformity, and growth retardation, happened during exposure period. Moreover, production of reactive oxygen species (ROS) significantly increased after DEN treatment. Then, alterations of the expression level of oxidative stress-related genes were observed in our results. To our knowledge, this is the first study concerning the effect of DEN on zebrafish. And from the information of our research, we speculated that development toxicity of DEN should be related to the excessive oxidative stress.
二乙基亚硝胺（DEN）存在于食物、水和日常用品中，被视为一种具有致癌性的毒物。DEN的发育毒性迄今鲜有报道。在本研究中，受精后6小时（hpf）的斑马鱼暴露于不同浓度的DEN中，以评估该化合物的胚胎毒性。结果表明，DEN对孵化率、心跳、体长和自发运动产生了负面影响。在暴露期间出现了畸形，包括脊索畸形、心包水肿、胚膜浑浊、尾部发育不全、卵黄囊畸形和生长迟缓。此外，DEN处理后活性氧（ROS）的产生显著增加。然后，我们的结果中观察到氧化应激相关基因表达水平的改变。据我们所知，这是第一项关于DEN对斑马鱼影响的研究。从我们的研究信息来看，我们推测DEN的发育毒性应该与过度的氧化应激有关。
