Zhang Cheng, Wang Jinhua, Dong Miao, Wang Jun, Du Zhongkun, Li Bing, Zhu Lusheng
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Taian, 271018, PR China.
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, Taian, 271018, PR China.
Chemosphere. 2018 Feb;192:348-353. doi: 10.1016/j.chemosphere.2017.10.169. Epub 2017 Nov 1.
Room-temperature ionic liquids, generally referred to ionic liquids (ILs), are "green solvents". Antioxidant responses and DNA damage in zebrafish livers exposed to 1-methyl-3-hexylimidazolium bromide ([Cmim]Br) were evaluated at various doses (5-40 mg/L) for a 28-day IL-exposure. A significant decrease of superoxide dismutase (SOD) activity was exhibited, and catalase (CAT) was inhibited at the highest dose (40 mg/L). Reactive oxygen species (ROS) levels were significantly promoted at most exposure interval times except for the dose of 5 mg/L on day 21 in male and days 21 and 28 in female. Malonaldehyde (MDA) contents remarkable increased exposed to [Cmim]Br. Besides, a notable increase was exhibited, which indicated an inducement of DNA damage with respect to control groups. Thus, we believed that [Cmim]Br causes oxidative stress and DNA damage in zebrafish. Gender differences were insignificant in almost all the tested biomarkers, thus, male and female zebrafish could be mixed at a ratio of 1:1 in the future evaluation. The present study may also provide basic toxicology information for IL evaluation to aquatic organisms.
室温离子液体，通常称为离子液体（ILs），是“绿色溶剂”。在28天的离子液体暴露实验中，评估了斑马鱼肝脏在不同剂量（5 - 40毫克/升）的1 - 甲基 - 3 - 己基咪唑溴盐（[Cmim]Br）作用下的抗氧化反应和DNA损伤情况。超氧化物歧化酶（SOD）活性显著降低，在最高剂量（40毫克/升）时过氧化氢酶（CAT）受到抑制。除了雄性在第21天以及雌性在第21天和第28天的5毫克/升剂量组外，在大多数暴露时间间隔内活性氧（ROS）水平都显著升高。暴露于[Cmim]Br后丙二醛（MDA）含量显著增加。此外，与对照组相比，DNA损伤有显著增加。因此，我们认为[Cmim]Br会导致斑马鱼产生氧化应激和DNA损伤。在几乎所有测试的生物标志物中，性别差异不显著，因此，在未来的评估中，雄性和雌性斑马鱼可以按1:1的比例混合。本研究也可为离子液体对水生生物的评估提供基础毒理学信息。
