Kaiser N
Horm Metab Res Suppl. 1979(8):30-3.
The use of lasers as light sources in IR spectroscopy allows an improvement in measuring sensitivity by a factor of about 100 as compared with the conventional technique. In addition, the monochromaticity of lasers appreciably improves the resolution. Combining lasers with an ATR plate acting as test prism gets rid of transmission cells without impairing the measuring sensitivity. It also considerably reduces the thermal load on the test sample; in vivo measurements on biological tissue can thus be made simply.
与传统技术相比,在红外光谱学中使用激光作为光源可使测量灵敏度提高约100倍。此外,激光的单色性显著提高了分辨率。将激光与用作测试棱镜的衰减全反射(ATR)板相结合,无需使用透射池,同时又不影响测量灵敏度。这也大大降低了测试样品的热负荷;因此,可以简单地对生物组织进行体内测量。