Katkov I I, Bolyukh V F, Sukhikh G T
Belgorod State National Research University, Belgorod, Russia.
CELLTRONIX, San Diego, California, USA.
Bull Exp Biol Med. 2018 Mar;164(4):530-535. doi: 10.1007/s10517-018-4027-8. Epub 2018 Mar 4.
Kinetic (dynamic) vitrification is a promising trend in cryopreservation of biological materials because it allows avoiding the formation of lethal intracellular ice and minimizes harmful effects of highly toxic penetrating cryoprotectants. A uniform cooling protocol and the same instruments can be used for practically all types of cells. In modern technologies, the rate of cooling is essentially limited by the Leidenfrost effect. We describe a novel platform for kinetic vitrification of biological materials KrioBlast that realizes hyper-fast cooling and allows overcoming the Leidenfrost effect. This opens prospects for creation of a novel technology of cell cryopreservation for reproductive and regenerative medicine.
动态玻璃化是生物材料冷冻保存领域一个很有前景的发展趋势，因为它能够避免细胞内形成致死性冰晶，并将高毒性渗透性冷冻保护剂的有害影响降至最低。几乎所有类型的细胞都可以采用统一的冷却方案和相同的仪器。在现代技术中，冷却速度主要受莱顿弗罗斯特效应的限制。我们描述了一种用于生物材料动态玻璃化的新型平台KrioBlast，它实现了超快速冷却，并能够克服莱顿弗罗斯特效应。这为生殖医学和再生医学创建一种新型细胞冷冻保存技术开辟了前景。
