Smirnova I P, Karimova E V, Shneider Yu A, Volina E G
Medical Faculty, Medical Institute, Peoples' Friendship University of Russia, Moscow, Russia.
Bull Exp Biol Med. 2018 Mar;164(4):459-461. doi: 10.1007/s10517-018-4012-2. Epub 2018 Mar 5.
Studies of the effects of Trichoderma harzianum Rifai F-180 culture fluid concentrate containing L-lysine-α-oxidase antitumor enzyme produced by the fungus and the homogenous enzyme, on ultrahazardous bacterium Acidovorax citrulli demonstrated the antibacterial activity of the concentrate. Trichoderma harzianum Rifai F-180 producing L-lysine-α-oxidase was cultured in a technological device at G. K. Skryabin Institute of Biochemistry and. Physiology of Microorganisms, Russian Academy of Sciences. Activity of L-lysine-α-oxidase in the resulted culture fluid concentrate was 0.54 U/ml, activity of the homogenous enzyme was 50 U/mg.
对含有由该真菌产生的L-赖氨酸-α-氧化酶抗肿瘤酶的哈茨木霉里氏木霉F-180培养液浓缩物以及该纯酶对超危险细菌西瓜嗜酸菌的作用研究表明了该浓缩物的抗菌活性。产生L-赖氨酸-α-氧化酶的哈茨木霉里氏木霉F-180在俄罗斯科学院G.K.斯克里亚宾微生物生物化学与生理学研究所的工艺装置中培养。所得培养液浓缩物中L-赖氨酸-α-氧化酶的活性为0.54 U/ml，纯酶的活性为50 U/mg。
