Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4O3, Canada.
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.
Angew Chem Int Ed Engl. 2018 Apr 16;57(17):4549-4553. doi: 10.1002/anie.201712903. Epub 2018 Mar 15.
We report a paper-based aptasensor platform that uses two reaction zones and a connecting bridge along with printed multifunctional bio/nano materials to achieve molecular recognition and signal amplification. Upon addition of analyte to the first zone, a fluorescently labelled DNA or RNA aptamer is desorbed from printed graphene oxide, rapidly producing an initial fluorescence signal. The released aptamer then flows to the second zone where it reacts with printed reagents to initiate rolling circle amplification, generating DNA amplicons containing a peroxidase-mimicking DNAzyme, which produces a colorimetric readout that can be read in an equipment-free manner or with a smartphone. The sensor was demonstrated using an RNA aptamer for adenosine triphosphate (a bacterial marker) and a DNA aptamer for glutamate dehydrogenase (Clostridium difficile marker) with excellent sensitivity and specificity. These targets could be detected in spiked serum or feacal samples, demonstrating the potential for testing clinical samples.
我们报告了一种基于纸的适体传感器平台，该平台使用两个反应区和一个连接桥以及印刷的多功能生物/纳米材料来实现分子识别和信号放大。在向第一区添加分析物后，荧光标记的 DNA 或 RNA 适体从印刷的氧化石墨烯上解吸，迅速产生初始荧光信号。然后，释放的适体流到第二区，在那里与印刷试剂反应，引发滚环扩增，生成含有过氧化物酶模拟 DNA 酶的 DNA 扩增子，产生比色读数，可以在无设备的情况下或使用智能手机进行读取。该传感器使用针对三磷酸腺苷（细菌标志物）的 RNA 适体和针对谷氨酸脱氢酶（艰难梭菌标志物）的 DNA 适体进行了演示，具有出色的灵敏度和特异性。这些靶标可以在添加的血清或粪便样本中检测到，表明该传感器具有检测临床样本的潜力。
