Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosensors (Basel). 2022 Nov 5;12(11):974. doi: 10.3390/bios12110974.
Herein, a paper-based glucose/air biofuel cell (BFC) was constructed and implemented for self-powered pesticide detection. Our developed paper-based chip relies on a hollow-channel to transport fluids rather than capillarity, which reduces analysis times as well as physical absorption. The gold nanoparticles (Au NPs) and carbon nanotubes (CNTs) were adapted to modify the paper fibers to fabricate the flexible conductive paper anode/cathode electrode (Au-PAE/CNT-PCE). Molecularly imprinted polymers (MIPs) using 2,4-dichlorophenoxyacetic acid (2,4-D) as a template were synthesized on Au-PAE for signal control. In the cathode, bilirubin oxidase (BOD) was used for the oxygen reduction reaction. Based on a competitive reaction between 2,4-D and glucose-oxidase-labeled 2,4-D (GOx-2,4-D), the amount of GOx immobilized on the bioanode can be simply tailored, thus a signal-off self-powered sensing platform was achieved for 2,4-D determination. Meanwhile, the coupling of the paper supercapacitor (PS) with the paper-based chip provides a simple route for signal amplification. Combined with a portable digital multi-meter detector, the amplified signal can be sensitively readout. Through rational design of the paper analytical device, the combination of BFC and PS provides a new prototype for constructing a low-cost, simple, portable, and sensitive self-powered biosensor lab-on-paper, which could be easily expanded in the field of clinical analysis and drug delivery.
本文构建并实施了一种基于纸张的葡萄糖/空气生物燃料电池（BFC），用于自供电农药检测。我们开发的基于纸张的芯片依赖于中空通道来输送流体，而不是毛细作用，从而缩短了分析时间和物理吸收。金纳米颗粒（Au NPs）和碳纳米管（CNTs）被用来修饰纸张纤维，以制造灵活的导电纸阳极/阴极电极（Au-PAE/CNT-PCE）。使用 2,4-二氯苯氧乙酸（2,4-D）作为模板的分子印迹聚合物（MIPs）被合成在 Au-PAE 上用于信号控制。在阴极，胆红素氧化酶（BOD）用于氧还原反应。基于 2,4-D 和葡萄糖氧化酶标记的 2,4-D（GOx-2,4-D）之间的竞争反应，可以简单地调整固定在生物阳极上的 GOx 的量，从而实现了基于信号关闭的自供电传感平台用于 2,4-D 的测定。同时，纸张超级电容器（PS）与纸张芯片的结合为信号放大提供了一种简单的途径。结合便携式数字多用表检测器，可以灵敏地读取放大后的信号。通过对纸张分析装置的合理设计，BFC 和 PS 的结合为构建低成本、简单、便携和敏感的自供电生物传感器纸芯片提供了一个新的原型，可以很容易地扩展到临床分析和药物输送领域。
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