Chen F Y, Fang X Y, Zhang H
Neurosurgery, Ningbo Second Hospital, Ningbo City, Zhejiang Province, China.
Facial Features, Yinzhou Second Hospital of Ningbo, Ningbo City, Zhejiang Province, China.
J Biol Regul Homeost Agents. 2018 Jan-Feb;32(1):133-138.
Secondary injury of brain tissue following cerebral infarction exhibits a complicated pathogenesis that is attributed to the induction of apoptosis and inflammatory response. The present study aimed to investigate the polydatin neuroprotective effects and their mode of action in cerebral ischemic injury. A total of 80 healthy adult male Sprague-Dawley rats were randomly divided into a Sham operated group, a permanent middle cerebral artery occlusion (MCAO) group, a Polydatin low dose group and a Polydatin high dose group. A total of 20 rats were used in each group. The right MCAO model of rats was established using the modified Longa suture method. The animals in the intervention group were injected intraperitoneally with 12.5mg/kg and/or 50mg/kg of polydatin following infarction. The expression levels of p53 and Notch 1 were measured by immunohistochemical techniques. The degree of left limb hemiplegia in the MCAO group was different compared with that of the low and high dose group of polydatin. The score for the neurological function in the high-dose group of polydatin was lower than that in the MCAO group (High vs MCAO: 2.4±0.31 vs 3.9±0.23, P less than0.05). The results indicated that high dose polydatin could reduce brain edema following cerebral infarction and improve the behavioral score in rats.
脑梗死后脑组织的继发性损伤表现出复杂的发病机制，这归因于细胞凋亡和炎症反应的诱导。本研究旨在探讨虎杖苷在脑缺血损伤中的神经保护作用及其作用方式。将80只健康成年雄性Sprague-Dawley大鼠随机分为假手术组、永久性大脑中动脉闭塞（MCAO）组、虎杖苷低剂量组和虎杖苷高剂量组。每组共20只大鼠。采用改良的Longa缝线法建立大鼠右侧MCAO模型。干预组动物在梗死后腹腔注射12.5mg/kg和/或50mg/kg虎杖苷。采用免疫组织化学技术检测p53和Notch 1的表达水平。MCAO组左下肢偏瘫程度与虎杖苷低、高剂量组不同。虎杖苷高剂量组神经功能评分低于MCAO组（高剂量组与MCAO组：2.4±0.31 vs 3.9±0.23，P＜0.05）。结果表明高剂量虎杖苷可减轻脑梗死大鼠脑水肿并改善其行为评分。
