a Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly (UP) , India.
b Directorate on Poultry Research , Rajendranagar, Hyderabad , Telangana , India.
Anim Biotechnol. 2019 Apr;30(2):113-117. doi: 10.1080/10495398.2018.1439845. Epub 2018 Mar 4.
The genetic polymorphism of Mx1 gene was explored in Indian chicken breeds. PCR-RFLP analysis in 102 bp fragment of partial intron 13 and partial exon 14 of Mx1 gene revealed two genotypes viz. RS and SS with two alleles viz. R and S both in Naked Neck and Tellicherry breeds of chicken. The homozygous genotype RR was not identified. When deduced amino acid sequences were compared, the asparagine amino acid was found to be substituted in "R" allele for serine in "S" allele. PCR-SSCP analysis of 284 bp fragment in 5'-UTR and partial promoter region revealed three genotypes viz. CC, CG, and CH with three different alleles viz. C, G, and H in Naked Neck breed of chicken and five genotypes viz. DI, JK, KK, KL, and KM with six different alleles viz. D, I, J, K, L, and M in Tellicherry breed of chicken. The homozygous genotypes viz. GG and HH in Naked Neck and DD, II, JJ, LL, and MM in Tellicherry chicken was not identified. The nucleotide substitution rate estimated to be in the range of 0.004-0.011. The identified genetic variation can be helpful for better insight to disease resistance property of the Mx1 gene.
本研究旨在探讨印度鸡种 Mx1 基因的遗传多态性。通过对 Mx1 基因第 13 号内含子部分片段和第 14 号外显子的 102bp 片段进行 PCR-RFLP 分析，在裸颈鸡和特立尼达鸡种中发现了两种基因型，即 RS 和 SS，两种等位基因，即 R 和 S。未鉴定出纯合基因型 RR。当比较推导的氨基酸序列时，发现“R”等位基因的天冬酰胺氨基酸被“S”等位基因的丝氨酸取代。5'-UTR 和部分启动子区 284bp 片段的 PCR-SSCP 分析显示，在裸颈鸡种中存在三种基因型，即 CC、CG 和 CH，三种不同的等位基因，即 C、G 和 H；在特立尼达鸡种中存在五种基因型，即 DI、JK、KK、KL 和 KM，六种不同的等位基因，即 D、I、J、K、L 和 M。裸颈鸡种中的 GG 和 HH 纯合基因型以及特立尼达鸡种中的 DD、II、JJ、LL 和 MM 纯合基因型未被鉴定。估计核苷酸取代率在 0.004-0.011 范围内。所鉴定的遗传变异可有助于更好地了解 Mx1 基因的抗病性。
