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骨癌痛大鼠背根神经节中脑源性神经营养因子的表达及其对疼痛行为的影响。

Expression of BDNF in dorsal root ganglion of rats with bone cancer pain and its effect on pain behavior.

作者信息

Huang Youqing

机构信息

Department of Pain, The Second Affiliated Hospital of Kunming Medical University, No.374, Dianmian Road, Kuming, Yunnan, 650101, P.R. China.

出版信息

J Musculoskelet Neuronal Interact. 2018 Mar 1;18(1):42-46.

PMID:29504577
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5881127/
Abstract

OBJECTIVE

To detect the expression of brain-derived neurotrophic factor (BDNF) in dorsal root ganglion (DRG) of rats with bone cancer pain, and to explore the effects of BDNF and anti-BDNF on pain behavior.

METHODS

40 SD rats were randomly divided into Sham group and Cancer group (n=20). Rats in Sham were injected with PBS into the tibia, while rats in Cancer group were injected with Walker 256 cells to construct rat tibial bone cancer pain model. Rats in Cancer group were further divided into physiological saline (NS) group and anti-BDNF group (n=10) to observe the effects of anti-BDNF on pain behavior in rats with bone cancer pain.

RESULTS

Expression level of BDNF and its receptor in DRG of Cancer group was significantly higher than that of Sham group at 3 weeks after inoculation (p⟨0.05). The expression level of BDNF mRNA in the Cancer group was significantly higher than that in the Sham group at 5d, 10d and 20d (p⟨0.05). Hindpaw withdrawal latency was significantly shorter in Cancer group than in Sham group (p⟨0.05). Hindpaw withdrawal mechanical threshold was significantly lower in Cancer group than in Sham group (p⟨0.05). Hindpaw withdrawal latency was significantly longer and hindpaw withdrawal mechanical threshold was significantly higher in anti-BDNF group than in NS group after the use of anti-BDNF (p⟨0.05).

CONCLUSION

BDNF can aggravate bone pain in rats, and anti-BDNF has obvious antinociceptive function in bone cancer pain.

摘要

目的

检测骨癌痛大鼠背根神经节（DRG）中脑源性神经营养因子（BDNF）的表达，探讨BDNF及抗BDNF对疼痛行为的影响。

方法

将40只SD大鼠随机分为假手术组和癌症组（n = 20）。假手术组大鼠胫骨内注射PBS，癌症组大鼠注射Walker 256细胞构建大鼠胫骨骨癌痛模型。癌症组大鼠进一步分为生理盐水（NS）组和抗BDNF组（n = 10），观察抗BDNF对骨癌痛大鼠疼痛行为的影响。

结果

接种后3周，癌症组DRG中BDNF及其受体的表达水平显著高于假手术组（p＜0.05）。癌症组BDNF mRNA在第5天、第10天和第20天的表达水平显著高于假手术组（p＜0.05）。癌症组大鼠的后爪缩足潜伏期明显短于假手术组（p＜0.05）。癌症组大鼠的后爪缩足机械阈值明显低于假手术组（p＜0.05）。使用抗BDNF后，抗BDNF组大鼠的后爪缩足潜伏期明显延长，后爪缩足机械阈值明显高于NS组（p＜0.05）。

结论

BDNF可加重大鼠骨痛，抗BDNF在骨癌痛中有明显的镇痛作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/5c095065dc2e/JMNI-18-042-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/8877fe6f81ab/JMNI-18-042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/aa4840d78e39/JMNI-18-042-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/bb3eb608c69e/JMNI-18-042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/95a09f8500e0/JMNI-18-042-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/dad115ff2d5c/JMNI-18-042-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/5c095065dc2e/JMNI-18-042-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/8877fe6f81ab/JMNI-18-042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/aa4840d78e39/JMNI-18-042-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/bb3eb608c69e/JMNI-18-042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/95a09f8500e0/JMNI-18-042-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/dad115ff2d5c/JMNI-18-042-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c768/5881127/5c095065dc2e/JMNI-18-042-g007.jpg

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