Institute of Biomaterials, Department of Materials Science and Engineering , University of Erlangen-Nuremberg , Cauerstrasse 6 , Erlangen 91058 , Germany.
Faculty of Engineering, Department of Mechanical, Materials and Manufacturing Engineering , University of Nottingham , Nottingham NG 7 2RD , United Kingdom.
ACS Appl Mater Interfaces. 2018 Apr 11;10(14):11529-11538. doi: 10.1021/acsami.8b01378. Epub 2018 Mar 29.
Structural and compositional modifications of metallic implant surfaces are being actively investigated to achieve improved bone-to-implant bonding. In this study, a strategy to modify bulk metallic surfaces by electrophoretic deposition (EPD) of short phosphate glass fibers (sPGF) is presented. Random and aligned orientation of sPGF embedded in a poly(acrylic acid) matrix is achieved by vertical and horizontal EPD, respectively. The influence of EPD parameters on the degree of alignment is investigated to pave the way for the fabrication of highly aligned sPGF structures in large areas. Importantly, the oriented sPGF structure in the coating, owing to the synergistic effects of bioactive composition and fiber orientation, plays an important role in directional cell migration and enhanced proliferation. Moreover, gene expression of MC3T3-E1 cells cultured with different concentrations of sPGF is thoroughly assessed to elucidate the potential stimulating effect of sPGF on osteogenic differentiation. This study represents an innovative exploitation of EPD to develop textured surfaces by orientation of fibers in the macroscale, which shows great potential for directional functionalization of metallic implants.
正在积极研究通过改变金属植入物表面的结构和组成来实现改善骨与植入物的结合。在这项研究中，提出了一种通过电泳沉积（EPD）短磷酸盐玻璃纤维（sPGF）来修饰整体金属表面的策略。通过垂直和水平 EPD 分别实现了 sPGF 在聚丙烯酸基质中的随机和定向排列。研究了 EPD 参数对定向程度的影响，为在大面积上制造高度定向的 sPGF 结构铺平了道路。重要的是，由于生物活性成分和纤维取向的协同作用，涂层中的定向 sPGF 结构在定向细胞迁移和增强增殖方面发挥着重要作用。此外，还彻底评估了用不同浓度 sPGF 培养的 MC3T3-E1 细胞的基因表达，以阐明 sPGF 对成骨分化的潜在刺激作用。这项研究代表了通过在宏观尺度上纤维取向来开发纹理表面的 EPD 的创新利用，这为金属植入物的定向功能化展示了巨大的潜力。
