Chen Qiang, Garcia Rosalina Pérez, Munoz Josemari, Pérez de Larraya Uxua, Garmendia Nere, Yao Qingqing, Boccaccini Aldo R
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg , Cauerstrasse 6, 91058 Erlangen, Germany.
CIDETEC, Parque Tecnológico de Miramón , Paseo Miramón 196, 20009 San Sebastian, Spain.
ACS Appl Mater Interfaces. 2015 Nov 11;7(44):24715-25. doi: 10.1021/acsami.5b07294. Epub 2015 Oct 27.
Surface functionalization of orthopedic implants is being intensively investigated to strengthen bone-to-implant contact and accelerate bone healing process. A hybrid coating, consisting of 45S5 bioactive glass (BG) individually wrapped and interconnected with fibrous cellulose nanocrystals (CNCs), is deposited on 316L stainless steel from aqueous suspension by a one-step electrophoretic deposition (EPD) process. Apart from the codeposition mechanism elucidated by means of zeta-potential and scanning electron microscopy measurements, in vitro characterization of the deposited CNCs-BG coating in simulated body fluid reveals an extremely rapid mineralization of BG particles on the coating (e.g., the formation of hydroxyapatite crystals layer after 0.5 day). A series of comparative trials and characterization methods were carried out to comprehensively understand the mineralization process of BG interacting with CNCs. Furthermore, key factors for satisfying the applicability of an implant coating such as coating composition, surface topography, and adhesion strength were quantitatively investigated as a function of mineralization time. Cell culture studies (using MC3T3-E1) indicate that the presence of CNCs-BG coating substantially accelerated cell attachment, spreading, proliferation, differentiation, and mineralization of extracellular matrix. This study has confirmed the capability of CNCs to enhance and regulate the bioactivity of BG particles, leading to mineralized CNCs-BG hybrids for improved bone implant coatings.
为增强骨与植入物的接触并加速骨愈合过程，人们正在深入研究骨科植入物的表面功能化。一种由45S5生物活性玻璃（BG）单独包裹并与纤维状纤维素纳米晶体（CNC）相互连接而成的混合涂层，通过一步电泳沉积（EPD）工艺从水悬浮液中沉积在316L不锈钢上。除了通过zeta电位和扫描电子显微镜测量阐明的共沉积机制外，在模拟体液中对沉积的CNCs-BG涂层进行的体外表征显示，涂层上的BG颗粒矿化极快（例如，0.5天后形成羟基磷灰石晶体层）。进行了一系列对比试验和表征方法，以全面了解BG与CNCs相互作用的矿化过程。此外，还定量研究了诸如涂层组成、表面形貌和粘附强度等满足植入物涂层适用性的关键因素与矿化时间的关系。细胞培养研究（使用MC3T3-E1）表明，CNCs-BG涂层的存在显著加速了细胞附着、铺展（伸展）、增殖、分化以及细胞外基质的矿化。这项研究证实了CNCs增强和调节BG颗粒生物活性的能力，从而形成矿化的CNCs-BG复合材料，用于改进骨植入物涂层。
