Kim Do-Young, Kim Kyeong-Min, Kim Eun-Jung, Jang Won-Gu
Department of Biotechnology, School of Engineering, Daegu University, Gyeongbuk, 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongbuk, 38453, Republic of Korea.
Research Institute of Anti-Aging, Daegu University, Gyeongbuk, 38453, Republic of Korea; Department of Immunology, Kyungpook National University School of Medicine, Daegu, 41944, Republic of Korea.
Biochem Biophys Res Commun. 2018 Apr 6;498(3):459-465. doi: 10.1016/j.bbrc.2018.02.209. Epub 2018 Mar 2.
The RNA-binding motif protein 3 (RBM3) belongs to a small group of proteins whose synthesis increases during hypothermia while global protein production is slowed down. Bone homeostasis is maintained by a balance between bone resorption and bone formation. Osteoblasts are key components of the bone and have an important role in bone remodeling cycle. However, hypothermia-induced RBM3 between osteoblasts remains unclear. At 32°C, expression of RBM3 and Runx2 was increased in a time-dependent manner and mineralization was also increased. RBM3 was also increased in a time-dependent manner under osteogenic conditions. Overexpression of RBM3 increased the expression of osteogenic genes such as Runx2 and OC. The osteogenic condition-induced expressions of RBM3, Runx2 and OC gene were decreased by RBM3 siRNA. Moreover, RBM3 promoted ERK and p38 phosphorylation. The inhibitor of ERK decreased the expression of Runx2 but did not affect the expression of RBM3. Taken together, these results demonstrate that RBM3 stimulates osteoblast differentiation via the ERK signaling pathway.
RNA结合基序蛋白3（RBM3）属于一小类蛋白质，其合成在体温过低时增加，而整体蛋白质产生则减缓。骨稳态通过骨吸收与骨形成之间的平衡得以维持。成骨细胞是骨骼的关键组成部分，在骨重塑周期中发挥重要作用。然而，体温过低诱导的成骨细胞间RBM3的作用仍不清楚。在32°C时，RBM3和Runx2的表达呈时间依赖性增加，矿化作用也增强。在成骨条件下，RBM3也呈时间依赖性增加。RBM3的过表达增加了Runx2和OC等成骨基因的表达。RBM3小干扰RNA降低了成骨条件诱导的RBM3、Runx2和OC基因的表达。此外，RBM3促进ERK和p38磷酸化。ERK抑制剂降低了Runx2的表达，但不影响RBM3的表达。综上所述，这些结果表明RBM3通过ERK信号通路刺激成骨细胞分化。
