Pastushkova L H, Kashirina D N, Brzhozovsky A G, Ivanisenko V A, Tiys E S, Kononikhin A S, Starodubtseva N L, Nikolaev E N, Binder H, Larina I M
Fiziol Cheloveka. 2017 Jan;43(1):89-96.
We investigated changes in the urine protein composition of healthy volunteers in controlled conditions during 105-day isolation (Mars-500 program) at different levels of salt consumption. We used newest proteomic techniques based on chromatography-mass spectrometry and various methods of bioinformatics including opoSOM. The period of observation can be divided into three intervals with different dynamics of protein excretion: early (week 1-6), intermediate (week 7-11) and late interval (week 12-15). We identified about 10 different groups of co-detected proteins, which are directly affected by periods with different-levels of salt consumption. We also determined the biological functions of these proteins, tissue specificity and signaling pathways that involve them.
我们研究了健康志愿者在105天隔离期间（火星-500计划），在不同盐摄入量的控制条件下尿液蛋白质组成的变化。我们使用了基于色谱-质谱联用的最新蛋白质组学技术以及包括opoSOM在内的各种生物信息学方法。观察期可分为三个阶段，蛋白质排泄呈现不同动态：早期（第1 - 6周）、中期（第7 - 11周）和后期（第12 - 15周）。我们鉴定出约10组共同检测到的蛋白质，它们直接受到不同盐摄入量阶段的影响。我们还确定了这些蛋白质的生物学功能、组织特异性以及涉及它们的信号通路。
