Cordier G
Biol Cell. 1986;58(2):147-50. doi: 10.1111/j.1768-322x.1986.tb00499.x.
Improvement in our knowledge in cellular biology is largely related to the use of new tools in quantitative cytology. Among them, flow cytometry was developed with numerous applications in the field of immunology including fundamental and applied research. Since its early beginning it has been associated with monoclonal antibodies to identify immuno-competent cells, to quantify changes in expression of surface determinants, to separate cells subsets prior to the test of their functional properties. Major advances gained using either single or dual-laser systems, multicolour fluorescence and computer facilities for multi-parametric analysis. Using this methodology it was possible to correlate analysis of cell cycle phases and membrane antigens expression. Applications have been developed for the analysis of new drugs in vitro, the evaluation of immunomodulating treatment and for clinical investigations.
我们在细胞生物学知识方面的进步在很大程度上与定量细胞学新工具的使用有关。其中，流式细胞术得到了发展，并在免疫学领域有众多应用，包括基础研究和应用研究。自其早期开始以来，它就与单克隆抗体相关联，用于识别免疫活性细胞、量化表面决定簇表达的变化、在测试细胞功能特性之前分离细胞亚群。使用单激光或双激光系统、多色荧光和用于多参数分析的计算机设备取得了重大进展。使用这种方法，可以将细胞周期阶段分析与膜抗原表达相关联。已经开发了用于体外新药分析、免疫调节治疗评估和临床研究的应用。
