SOX2抑制胃癌细胞的增殖和转移，通过下调CCND1和PARP促进细胞凋亡。
SOX2 inhibits cell proliferation and metastasis, promotes apoptotic by downregulating CCND1 and PARP in gastric cancer.
作者信息
Luo Jianfei, Yan Ruicheng, He Xiaobo, He Jie
机构信息
Department of Gastrointestinal Surgery, Renmin Hospital of Wuhan UniversityWuhan 430060, Hubei Province, China.
出版信息
Am J Transl Res. 2018 Feb 15;10(2):639-647. eCollection 2018.
Abstract
Inconsistent results of Sex-determining region Y-box2 (SOX2) expression have been reported in gastric cancer (GC) before. Our recent studies showed that SOX2 was significantly downregulated in GC cells compared with GES-1 at both mRNA and protein level. Transfected with pcDNA3.1-SOX2 resulted in enforced expression of SOX2 at mRNA and protein levels compared with NC group in undifferentiated cell lines including HGC27 and BGC823. MTT assay showed that exogenous expressed SOX2 suppressed cell proliferation. FC analysis revealed that SOX2-overexpressing cells exhibited cell-cycle arrest and apoptosis. Transwell assay showed the anti-metastatic effect of SOX2 in GC cells. The subsequent results suggested CCND1 and PARP were downregulated in SOX2 overexpressed GC cells, and were responsible for the SOX2-induced anticancer effects. Thus, SOX2 proved to be an expected biomarker in GC diagnosis.
摘要
此前已有报道称，性别决定区Y盒2（SOX2）在胃癌（GC）中的表达结果不一致。我们最近的研究表明，与GES-1相比，GC细胞中SOX2在mRNA和蛋白质水平均显著下调。在包括HGC27和BGC823在内的未分化细胞系中，与NC组相比，用pcDNA3.1-SOX2转染导致SOX2在mRNA和蛋白质水平上的表达增强。MTT分析表明，外源性表达的SOX2抑制细胞增殖。流式细胞术分析显示，过表达SOX2的细胞表现出细胞周期停滞和凋亡。Transwell分析显示SOX2对GC细胞具有抗转移作用。随后的结果表明，在SOX2过表达的GC细胞中，细胞周期蛋白D1（CCND1）和聚（ADP-核糖）聚合酶（PARP）下调，这是SOX2诱导抗癌作用的原因。因此，SOX2被证明是GC诊断中一个有前景的生物标志物。
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