白细胞介素-21 rs2055979和白细胞介素-21受体rs3093390基因变异与丙型肝炎病毒慢性感染

Interleukin-21 rs2055979 and Interleukin-21 receptor rs3093390 genetic variants and hepatitis C virus chronic infection.

作者信息

Shoraka Shahrzad, Mohebbi Seyed Reza, Hosseini Seyed Masoud, Hosseini Razavi Armin, Ghaemi Amir, Kazemian Shabnam, Rostami-Nejad Mohammad

机构信息

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

出版信息

Gastroenterol Hepatol Bed Bench. 2017 Winter;10(Suppl1):S154-S160.

PMID:29511486

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5838195/

Abstract

AIM

The aim of this case-control study was to investigate association of G/T IL-21 (rs2055979) and C/T IL-21R (rs3093390) gene polymorphisms with chronic hepatitis C virus in Iranian Patients.

BACKGROUND

Interleukin 21 (IL-21) has a significant function in the regulation of cellular immune responses. Its exclusive receptor, IL-21R, expressed on the surface of T, B and NK cells and is important for the proliferation and differentiation of these immune cells. Hence, it was suggested to be involved in response to viral infections.

METHODS

This study follows a case-control study design and blood samples were collected from 290 patients with chronic HCV and 290 controls for both genes. Genomic DNA was extracted and then for each position, SNP was genotyped by the dedicated PCR and restriction fragment length polymorphism (RFLP) method. The results were analyzed by SPSS software using logistic regression and Chi-square tests.

RESULTS

Genotype frequencies of GG, GT and TT in IL21 gene (rs3093390) were found to be 27.6%, 48.3%, 24.1% and 25.2%, 55,5%, 19,3% respectively in HCV infected patients and control group. For IL21R gene (rs2055979) genotype frequencies of CC, CT and TT were 63.8%, 31.4%, 4.8% and 61.4%, 29.7%, 9.0% respectively in HCV infected patients and control group. P values for genotype and allele frequencies were p=0.188, p=0.769 for IL21 gene, and p=0.144, p=0.179 for IL21R gene respectively.

CONCLUSION

As a result, there is no evidence for an association between IL-21 (rs2055979) and IL-21R (rs3093390) gene polymorphisms with chronic hepatitis C virus in Iranian Patients.

摘要

目的

本病例对照研究旨在调查伊朗患者中G/T白细胞介素-21（rs2055979）和C/T白细胞介素-21受体（rs3093390）基因多态性与慢性丙型肝炎病毒的关联。

背景

白细胞介素21（IL-21）在细胞免疫反应调节中具有重要作用。其唯一的受体IL-21R在T细胞、B细胞和NK细胞表面表达，对这些免疫细胞的增殖和分化至关重要。因此，有人认为它参与了对病毒感染的反应。

方法

本研究采用病例对照研究设计，收集了290例慢性丙型肝炎患者和290例对照者的血液样本用于这两个基因的检测。提取基因组DNA，然后针对每个位点，通过专用的聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）方法对单核苷酸多态性（SNP）进行基因分型。使用SPSS软件通过逻辑回归和卡方检验对结果进行分析。

结果

在丙型肝炎病毒感染患者和对照组中，IL21基因（rs3093390）的GG、GT和TT基因型频率分别为27.6%、48.3%、24.1%和25.2%、55.5%、19.3%。对于IL21R基因（rs2055979），丙型肝炎病毒感染患者和对照组中CC、CT和TT基因型频率分别为63.8%、31.4%、4.8%和61.4%、29.7%、9.0%。基因型和等位基因频率的P值分别为：IL21基因p = 0.188，p = 0.769；IL21R基因p = 0.144，p = 0.179。