Zhai Laiyuan, Zheng Tianqing, Wang Xinyu, Wang Yun, Chen Kai, Wang Shu, Wang Yun, Xu Jianlong, Li Zhikang
Rice Research Institute, Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education, Shenyang, 110866, China.
Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, 12# South Zhong-Guan-Cun Street, Haidain District, Beijing, 100081, China.
Rice (N Y). 2018 Mar 6;11(1):13. doi: 10.1186/s12284-018-0204-7.
The vascular bundle especially in the peduncle is one of crucial limiting factors of rice yield, and it determines how plants efficiently transport photosynthetic products, mineral nutrients and water from leaf and root to panicle. However, the genetic base of rice vascular bundle related traits in the peduncle still remains unknown.
The 423 panel showed substantial natural variations of peduncle vascular bundle. In total, 48 quantitative trait loci/locus (QTL) affecting the eight traits were identified throughout the genome by applying a significance threshold of P < 1.0 × 10. Combined determining linkage disequilibrium (LD) blocks associated with significant SNPs and haplotype analyses allowed us to shortlist six candidate genes for four important QTL regions affecting the peduncle vascular bundle traits, including one cloned gene (NAL1) and three newly identified QTL (qLVN6, qSVN7, and qSVA8.1). Further the most likely candidate genes for each important QTL were also discussed based on functional annotation.
Genetic base on peduncle vascular bundle related traits in rice was systematically dissected, and most likely candidate genes of the known gene NAL1 and the three newly identified QTL (qLVN6, qSVN7, and qSVA8.1) were analyzed. The results provided valuable information for future functional characterization and rice breeding for high yield through optimizing transportation efficiency of photosynthetic products by marker-assisted selection.
维管束尤其是穗颈维管束是限制水稻产量的关键因素之一，它决定了植株如何有效地将光合产物、矿质养分和水分从叶片和根系运输到穗部。然而，水稻穗颈维管束相关性状的遗传基础仍不清楚。
423份材料的穗颈维管束表现出显著的自然变异。通过应用P < 1.0×10的显著性阈值，在全基因组中总共鉴定出48个影响8个性状的数量性状基因座（QTL）。结合确定与显著单核苷酸多态性（SNP）相关的连锁不平衡（LD）区段和单倍型分析，我们筛选出了影响穗颈维管束性状的4个重要QTL区域的6个候选基因，包括1个已克隆基因（NAL1）和3个新鉴定的QTL（qLVN6、qSVN7和qSVA8.1）。此外，还根据功能注释讨论了每个重要QTL最可能的候选基因。
系统剖析了水稻穗颈维管束相关性状的遗传基础，并分析了已知基因NAL1和3个新鉴定QTL（qLVN6、qSVN7和qSVA8.1）最可能的候选基因。这些结果为未来通过标记辅助选择优化光合产物运输效率进行功能鉴定和高产水稻育种提供了有价值的信息。
