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水稻(Oryza sativa L.)穗颈直径的QTL定位及相关基因的代换作图

Characterization of QTLs for diameter in panicle neck and substitution mapping of and in rice ( L.).

作者信息

Nguyen Ha Thi Le, Yoshiura Ami, Zheng Shao-Hui, Fujita Daisuke

机构信息

The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-8580, Japan.

Forest Science Institute of South Vietnam, 1 Pham Van Hai, Tan Binh District, Ho Chi Minh City, Viet Nam.

出版信息

Breed Sci. 2024 Sep;74(4):337-343. doi: 10.1270/jsbbs.23076. Epub 2024 Aug 14.

DOI:10.1270/jsbbs.23076
PMID:39872323
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11769591/
Abstract

The vascular bundle system in the panicle neck of rice ( L.) connects the culm to the panicle and transports assimilates. The number of vascular bundles in the panicle neck (VBN) is correlated with the diameter of the panicle neck (DPN), but there are few reported QTLs for DPN. We conducted quantitative trait locus (QTL) analysis using recombinant inbred lines (RILs) derived from a cross between 'Asominori' and 'IR24' and detected three QTLs-, , and -on chromosomes 5, 6, and 11. The , , and were in the same position as the QTLs for VBN reported in previous studies. Within the RILs, there was a significant positive correlation between DPN and VBN. In segregating populations, each QTL had a distinct effect on both values. Analysis of chromosome segment substitution lines showed that and affected DPN and affected VBN. Through substitution mapping, we narrowed down the region of and to 960 kbp between KNJ8 Indel385 and RM18926, and the region of to 750 kbp between C5 Indel5756 and KNJ8 Indel493. Due to the weak effect of in the 'IR24' genetic background, the location of could not be determined.

摘要

水稻(Oryza sativa L.)穗颈中的维管束系统连接茎秆和穗部,并运输同化物。穗颈维管束数量(VBN)与穗颈直径(DPN)相关,但关于DPN的数量性状基因座(QTL)报道较少。我们利用‘Asominori’和‘IR24’杂交衍生的重组自交系(RIL)进行了QTL分析,在第5、6和11号染色体上检测到3个QTL——qPND5、qPND6和qPND11。qPND5、qPND6和qPND11与先前研究报道的VBN的QTL位于相同位置。在RIL群体中,DPN与VBN之间存在显著正相关。在分离群体中,每个QTL对这两个值都有不同的影响。染色体片段代换系分析表明,qPND5和qPND6影响DPN,qPND11影响VBN。通过代换定位,我们将qPND5和qPND6的区域缩小到KNJ8 Indel385和RM18926之间的960 kbp,将qPND11的区域缩小到C5 Indel5756和KNJ8 Indel493之间的750 kbp。由于在‘IR24’遗传背景下qPND11的效应较弱,无法确定其位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92f/11769591/e7df2b6ee1c2/74_337-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92f/11769591/3f048a5758b6/74_337-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92f/11769591/e7df2b6ee1c2/74_337-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92f/11769591/3f048a5758b6/74_337-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f92f/11769591/e7df2b6ee1c2/74_337-g002.jpg

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本文引用的文献

1
Identification and characterization of stable QTLs for vascular bundle number at the panicle neck in rice ( L.).水稻穗颈维管束数目稳定QTL的鉴定与特征分析
Breed Sci. 2023 Sep;73(4):365-372. doi: 10.1270/jsbbs.23013. Epub 2023 Sep 9.
2
Genetic basis of vascular bundle variations in rice revealed by genome-wide association study.基因组关联研究揭示了水稻维管束变异的遗传基础。
Plant Sci. 2021 Jan;302:110715. doi: 10.1016/j.plantsci.2020.110715. Epub 2020 Oct 15.
3
Multiple areas investigation reveals the genes related to vascular bundles in rice.
多区域调查揭示了水稻中与维管束相关的基因。
Rice (N Y). 2019 Mar 21;12(1):17. doi: 10.1186/s12284-019-0278-x.
4
QTL mapping and candidate gene analysis of peduncle vascular bundle related traits in rice by genome-wide association study.基于全基因组关联研究的水稻穗颈维管束相关性状的QTL定位及候选基因分析
Rice (N Y). 2018 Mar 6;11(1):13. doi: 10.1186/s12284-018-0204-7.
5
Genome-wide indel markers shared by diverse Asian rice cultivars compared to Japanese rice cultivar 'Koshihikari'.与日本水稻品种“越光”相比,不同亚洲水稻品种共有的全基因组插入缺失标记。
Breed Sci. 2015 Jun;65(3):249-56. doi: 10.1270/jsbbs.65.249. Epub 2015 Jun 1.
6
NAL1 allele from a rice landrace greatly increases yield in modern indica cultivars.NAL1 等位基因来源于一个水稻地方品种,可极大提高现代籼稻品种的产量。
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20431-6. doi: 10.1073/pnas.1310790110. Epub 2013 Dec 2.
7
The plant vascular system: evolution, development and functions.植物维管系统:进化、发育与功能。
J Integr Plant Biol. 2013 Apr;55(4):294-388. doi: 10.1111/jipb.12041.
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Yield-related QTLs and their applications in rice genetic improvement.与产量相关的 QTLs 及其在水稻遗传改良中的应用。
J Integr Plant Biol. 2012 May;54(5):300-11. doi: 10.1111/j.1744-7909.2012.01117.x.
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A gene controlling the number of primary rachis branches also controls the vascular bundle formation and hence is responsible to increase the harvest index and grain yield in rice.一个控制一级穗分枝数的基因也控制着维管束的形成,因此能够提高水稻的收获指数和产量。
Theor Appl Genet. 2010 Mar;120(5):875-93. doi: 10.1007/s00122-009-1218-8. Epub 2009 Nov 22.
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Plant Physiol. 2008 Aug;147(4):1947-59. doi: 10.1104/pp.108.118778. Epub 2008 Jun 18.