职业性接触沥青烟可引起道路铺筑工人的氧化 DNA 损伤。
Occupational exposure to asphalt fume can cause oxidative DNA damage among road paving workers.
机构信息
Department of Biochemistry, Yıldırım Beyazıt University, Ankara, Turkey.
Department of Pharmacology, Occupational and Environmental Diseases Hospital, Ankara, Turkey.
出版信息
Am J Ind Med. 2018 Jun;61(6):471-476. doi: 10.1002/ajim.22830. Epub 2018 Mar 7.
OBJECTIVES
We designed the present study to determine the effect of occupational exposure to asphalt fumes on oxidative status and DNA damage in road paving workers.
METHODS
Sixty road paving workers exposed to asphalt fumes and forty non-exposed control subjects were recruited. Occupational exposure to PAHs was assessed by urinary 1-hydroxypyrene (1-OHP) excretion. Serum thiol disulfide homeostasis (TDH), total oxidant status (TOS) and total antioxidant status (TAS) and urinary 8-hydro-deoxyguanosine (8-OH-dG) level were evaluated by automated colourimetric method.
RESULTS
The urinary concentrations of 1-OHP and 8-OH-dG were significantly higher in the exposed group than in the control group (P < 0.001). Disulfide/thiol ratio, TOS, and TAS were also significantly higher for the asphalt workers. A positive correlation existed between urinary 1-OHP and 8-OH-dG, TOS and TAS.
CONCLUSION
Study results indicate that exposure to PAHs induces oxidative stress and causes genotoxic effects in asphalt workers.
目的
本研究旨在确定接触沥青烟雾对道路铺设工人氧化状态和 DNA 损伤的影响。
方法
招募了 60 名接触沥青烟雾的道路铺设工人和 40 名非接触对照者。通过尿 1-羟基芘（1-OHP）排泄来评估多环芳烃（PAHs）的职业暴露。通过自动比色法评估血清硫醇二硫化物平衡（TDH）、总氧化剂状态（TOS）和总抗氧化状态（TAS）以及尿 8-羟基脱氧鸟苷（8-OH-dG）水平。
结果
暴露组的尿 1-OHP 和 8-OH-dG 浓度明显高于对照组（P<0.001）。二硫键/巯基比、TOS 和 TAS 也明显更高。尿 1-OHP 与 8-OH-dG、TOS 和 TAS 之间存在正相关。
结论
研究结果表明，接触多环芳烃会诱导氧化应激，并导致沥青工人产生遗传毒性作用。
Zotero 插件