Department of Medical Biochemistry, Faculty of Medicine, Ankara Yıldırım Beyazıt Universty, Ankara , Turkey
Department of Medical Biochemistry, Ankara City Hospital , Ankara, Turkey
Turk J Med Sci. 2020 Nov 3;50(SI-2):1728-1738. doi: 10.3906/sag-2003-64.
Dynamic thiol-disulfide homeostasis (TDH) is a new area has begun to attract more scrutiny. Dynamic TDH is reversal of thiol oxidation in proteins and represents the status of thiols (-SH) and disulfides (-S-S-). Organic compounds containing the sulfhydryl group is called thiol, composed of sulfur and hydrogen atoms. Disulfides are the most important class of dynamic, redox responsive covalent bonds build in between two thiol groups. For many years, thiol levels were analyzed by several methods. During last years, measurements of disulfide levels have been analyzed by a novel automated method, developed by Erel and Neselioglu. In this method, addition to thiol (termed as native thiol) levels, disulfide levels were also measured and sum of native thiol and disulfide levels were termed as total thiol. Therefore, TDH was begun to be understood in organism. In healthy humans, TDH is maintained within a certain range. Dysregulated dynamic TDH has been implicated several disorders with unknown etiology. A growing body of evidence has demonstrated that the thiol-disulfide homeostasis is involved in variety diseases, such as diabetes mellitus, hypertension, nonsmall cell lung cancer, familial Mediterranean fever (FMF), inflammatory bowel diseases, occupational diseases, gestational diabetes mellitus and preeclampsia. These results may elucidate some pathogenic mechanism or may be a predictor indicating diagnostic clue, prognostic marker or therapeutic sign. In conclusion, protection of the thiol-disulfide homeostasis is of great importance for the human being. Evidence achieved so far has proposed that thiol-disulfide homeostasis is an important issue needs to elucidate wholly.
动态硫醇-二硫键动态平衡（TDH）是一个新的领域，开始引起更多的关注。动态 TDH 是蛋白质中硫醇氧化的逆转，代表了硫醇（-SH）和二硫键（-S-S-）的状态。含有巯基的有机化合物称为硫醇，由硫和氢原子组成。二硫键是最重要的一类动态、氧化还原响应的共价键，由两个巯基之间形成。多年来，硫醇水平一直通过多种方法进行分析。在过去的几年中，二硫键水平的测量一直通过一种由 Erel 和 Neselioglu 开发的新的自动化方法进行分析。在这种方法中，除了硫醇（称为天然硫醇）水平外，还测量了二硫键水平，并且天然硫醇和二硫键水平的总和被称为总硫醇。因此，在生物体中开始理解 TDH。在健康的人类中，TDH 保持在一定的范围内。失调的动态 TDH 与许多病因不明的疾病有关。越来越多的证据表明，硫醇-二硫键动态平衡参与多种疾病，如糖尿病、高血压、非小细胞肺癌、家族性地中海热（FMF）、炎症性肠病、职业病、妊娠期糖尿病和子痫前期。这些结果可能阐明一些发病机制，也可能是指示诊断线索、预后标志物或治疗标志的预测因子。总之，保护硫醇-二硫键动态平衡对人类非常重要。迄今为止所获得的证据表明，硫醇-二硫键动态平衡是一个需要全面阐明的重要问题。
