促进大肠杆菌中同源重组的遗传功能：对λ噬菌体中倒位的研究
Genetic functions promoting homologous recombination in Escherichia coli: a study of inversions in phage lambda.
作者信息
Ennis D G, Amundsen S K, Smith G R
出版信息
Genetics. 1987 Jan;115(1):11-24. doi: 10.1093/genetics/115.1.11.
Abstract
We have studied homologous recombination in a derivative of phage lambda containing two 1.4-kb repeats in inverted orientation. Inversion of the intervening 2.5-kb segment occurred efficiently by the Escherichia coli RecBC pathway but markedly less efficiently by the lambda Red pathway or the E. coli RecE or RecF pathways. Inversion by the RecBCD pathway was stimulated by Chi sites located to the right of the invertible segment; this stimulation decreased exponentially by a factor of about 2 for each 2.2 kb between the invertible segment and the Chi site. In addition to RecA protein and RecBCD enzyme, inversion by the RecBC pathway required single-stranded DNA binding protein, DNA gyrase, DNA polymerase I and DNA ligase. Inversion appeared to occur either intra- or intermolecularly. These results are discussed in the framework of a current molecular model for the RecBC pathway of homologous recombination.
摘要
我们研究了噬菌体λ的一个衍生物中的同源重组，该衍生物含有两个反向排列的1.4 kb重复序列。中间2.5 kb片段的倒位通过大肠杆菌RecBC途径高效发生，但通过λ Red途径或大肠杆菌RecE或RecF途径发生的效率明显较低。RecBCD途径介导的倒位受到位于可倒位片段右侧的Chi位点的刺激；对于可倒位片段与Chi位点之间每2.2 kb的距离，这种刺激以约2的倍数呈指数下降。除了RecA蛋白和RecBCD酶外，RecBC途径介导的倒位还需要单链DNA结合蛋白、DNA旋转酶、DNA聚合酶I和DNA连接酶。倒位似乎发生在分子内或分子间。这些结果在同源重组RecBC途径的当前分子模型框架内进行了讨论。
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