Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang, Guizhou, P. R. China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, P. R. China.
PLoS One. 2018 Mar 7;13(3):e0192367. doi: 10.1371/journal.pone.0192367. eCollection 2018.
Nitric oxide (NO) is an important signaling molecule with diverse physiological functions in plants. It is therefore important to characterize the downstream genes and signal transduction networks modulated by NO. Here, we identified 1,932 differentially expressed genes (DEGs) responding to NO in upland cotton using high throughput tag sequencing. The results of quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 25 DEGs showed good consistency. Gene Ontology (GO) and KEGG pathway were analyzed to gain a better understanding of these DEGs. We identified 157 DEGs belonging to 36 transcription factor (TF) families and 72 DEGs related to eight plant hormones, among which several TF families and hormones were involved in stress responses. Hydrogen peroxide and malondialdehyde (MDA) contents were increased, as well related genes after treatment with sodium nitroprusside (SNP) (an NO donor), suggesting a role for NO in the plant stress response. Finally, we compared of the current and previous data indicating a massive number of NO-responsive genes at the large-scale transcriptome level. This study evaluated the landscape of NO-responsive genes in cotton and identified the involvement of NO in the stress response. Some of the identified DEGs represent good candidates for further functional analysis in cotton.
一氧化氮（NO）是一种重要的信号分子，在植物中有多种生理功能。因此，表征 NO 调节的下游基因和信号转导网络非常重要。在这里，我们使用高通量标签测序技术鉴定了 1932 个陆地棉响应 NO 的差异表达基因（DEGs）。25 个 DEGs 的定量实时聚合酶链反应（qRT-PCR）分析结果显示出良好的一致性。对这些 DEGs 进行基因本体论（GO）和 KEGG 途径分析，以更好地理解这些 DEGs。我们鉴定了 157 个属于 36 个转录因子（TF）家族的 DEGs 和 72 个与八种植物激素相关的 DEGs，其中一些 TF 家族和激素参与了应激反应。用硝普钠（NO 供体）处理后，过氧化氢和丙二醛（MDA）含量增加，相关基因也增加，表明 NO 参与了植物的应激反应。最后，我们比较了当前和以前的数据，表明在大规模转录组水平上存在大量的 NO 响应基因。本研究评估了棉花中 NO 响应基因的概况，并确定了 NO 参与应激反应。鉴定出的一些 DEGs 是棉花进一步功能分析的良好候选基因。
