Lindemann G, Nylen M U
Scand J Dent Res. 1979 Oct;87(5):381-9. doi: 10.1111/j.1600-0722.1979.tb00697.x.
Femurs and tibias of normal rats were fixed in 4% unbuffered solutions of glutaraldehyde or formaldehyde containing 1% NaF. Following washing, radiographically controlled demineralization was performed in 3.25% HNO3. Light microscopy and transmission electron microscopy revealed the presence of so-called fluoride granules in bone trabeculae, cartilage, cartilage remnants, bone cells, chondrocytes and in the bone marrow. Selected area diffraction indicated that the fluoride granules contained CaF2. X-ray diffraction analysis of non-demineralized NaF-fixed bones were negative for CaF2. The results are discussed and related to precipitation artifacts reported in demineralized hard tissues and to similar CaF2-containing granules observed in demineralized bones and incisors of rats given high doses of NaF in diet or drinking water.
将正常大鼠的股骨和胫骨固定于含1%氟化钠的4%戊二醛或甲醛非缓冲溶液中。冲洗后，在3.25%硝酸中进行放射学控制的脱矿处理。光学显微镜和透射电子显微镜检查显示，在骨小梁、软骨、软骨残余物、骨细胞、软骨细胞及骨髓中存在所谓的氟化物颗粒。选区衍射表明，氟化物颗粒含有氟化钙。未脱矿的氟化钠固定骨的X射线衍射分析对氟化钙呈阴性。对结果进行了讨论，并与脱矿硬组织中报道的沉淀假象以及在饮食或饮用水中摄入高剂量氟化钠的大鼠的脱矿骨和切牙中观察到的类似含氟化钙颗粒相关联。
