Department of Biosystems and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
Department of Biosystems and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
Gene. 2019 Feb 5;684:82-94. doi: 10.1016/j.gene.2018.10.055. Epub 2018 Oct 22.
Although rye is one of the most cold-tolerant species among temperate cereals, its huge and complex genome has prevented us from identifying agronomically useful genes. However, advances in high-throughput sequencing technology are making it increasingly possible to investigate its genome. The C-repeat binding factor (CBF) gene family controls cold tolerance in plants and its members are well conserved among eudicots and monocots, among which there are diverse homologs. Despite its large genome, only a small number of CBF genes have been identified in rye. In this study, we explored high-throughput sequencing data of the rye genome and identified 12 novel CBF genes. Sequence analyses revealed that these genes contain signature sequences of the CBF family. Chromosomal localization of the genes by PCR using wheat-rye addition lines showed that most of these are located on the long arm of chromosome 5, but also on the long arm of chromosomes 2 and 6. On the basis of comparative analyses of CBF family members in the Triticeae, CBF proteins were divided into several groups according to phylogenetic relationship and conserved motifs. Light is essential to fully induce CBF gene expression and there is specificity in the response to different types of abiotic stresses in ScCBF genes. The results of our study will assist investigations of CBF genes in the Triticeae and the mechanism of cold tolerance through the CBF-dependent pathway in plants.
尽管黑麦是温带谷类作物中最耐寒的物种之一，但它庞大而复杂的基因组却阻碍了我们识别具有农艺用途的基因。然而，高通量测序技术的进步使得研究其基因组变得越来越可行。C-重复结合因子（CBF）基因家族控制着植物的耐寒性，其成员在真双子叶植物和单子叶植物中高度保守，其中存在着多样化的同源物。尽管黑麦基因组庞大，但仅鉴定出少数几个 CBF 基因。在这项研究中，我们探索了黑麦基因组的高通量测序数据，并鉴定出了 12 个新的 CBF 基因。序列分析表明，这些基因包含 CBF 家族的特征序列。使用小麦-黑麦添加系通过 PCR 对基因进行染色体定位显示，这些基因大多数位于 5 号染色体的长臂上，但也位于 2 号和 6 号染色体的长臂上。根据三核体中 CBF 家族成员的比较分析，根据系统发育关系和保守基序，CBF 蛋白被分为几个组。光照是完全诱导 CBF 基因表达所必需的，而 ScCBF 基因对不同类型的非生物胁迫的反应具有特异性。我们的研究结果将有助于研究三核体中的 CBF 基因以及植物中通过 CBF 依赖途径的耐寒性机制。
