Programa Regional de Doctorado en Biotecnología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, 80010, Culiacán, Sin., Mexico.
Maestría en Ciencia y Tecnología de Alimentos, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, 80010, Culiacán, Sin., Mexico.
Plant Foods Hum Nutr. 2018 Jun;73(2):122-129. doi: 10.1007/s11130-018-0661-6.
Chickpea (Cicer arietinum L.) genotypes, nine kabuli from Mexico and 9 desi from other countries, were investigated for their phenolic profiles and antioxidant activity (AA). Phenolics in methanol extracts (ME) were analyzed by ultra-performance liquid chromatography coupled to diode array detection and mass spectrometry (UPLC-DAD-MS), whereas the AA was measured as Trolox equivalents (TE) by ABTS, DPPH and FRAP methods. Twenty phenolic compounds were identified in the ME and their levels showed a great variability among the chickpea genotypes. Phenolic acids and flavonoids were the most abundant compounds in kabuli and desi genotypes, respectively. The AA values (μmol TE/ 100 g dw) by ABTS (278-2417), DPPH (52-1650), and FRAP (41-1181) were mainly associated with the content of sinapic acid hexoside, gallic acid, myricetin, quercetin, catechin, and isorhamnetin, suggesting they are the main compounds responsible for the AA. The sum of the AA obtained for standards of these compounds evaluated at the concentration found in the extracts accounted for 34.3, 69.8, and 47.0% of the AA in the extract by ABTS, DPPH, and FRAP, respectively. In the AA by DPPH, most of the mixtures of these compounds resulted in synergistic interactions. Three desi genotypes with black seeds (ICC 4418, ICC 6306, and ICC 3761) showed the highest AA and flavonoids content, whereas the most promising kabuli genotypes were Surutato 77, Bco. Sin. 92, and Blanoro that showed the highest values of phenolic acids. These genotypes represent good sources of antioxidants for the improvement of nutraceutical properties in chickpea.
鹰嘴豆（Cicer arietinum L.）品种，9 个来自墨西哥的卡布里亚种和 9 个来自其他国家的德西亚种，其酚类物质谱和抗氧化活性（AA）进行了研究。甲醇提取物（ME）中的酚类物质通过超高效液相色谱与二极管阵列检测和质谱联用（UPLC-DAD-MS）进行分析，而 AA 则通过 ABTS、DPPH 和 FRAP 方法测量为 Trolox 当量（TE）。在 ME 中鉴定出 20 种酚类化合物，其水平在鹰嘴豆品种间表现出很大的可变性。酚酸和类黄酮分别是卡布里亚种和德西亚种中含量最丰富的化合物。ABTS（278-2417）、DPPH（52-1650）和 FRAP（41-1181）的 AA 值（μmol TE/100 g dw）主要与芥子酸己糖苷、没食子酸、杨梅素、槲皮素、儿茶素和异鼠李素的含量有关，表明它们是 AA 的主要贡献化合物。用提取物中发现的浓度评估这些化合物的标准品的 AA 总和分别占 ABTS、DPPH 和 FRAP 提取物 AA 的 34.3%、69.8%和 47.0%。在 DPPH 的 AA 中，这些化合物的大多数混合物都表现出协同作用。三个具有黑色种子的德西亚种（ICC 4418、ICC 6306 和 ICC 3761）表现出最高的 AA 和类黄酮含量，而最有前途的卡布里亚种是 Surutato 77、Bco. Sin. 92 和 Blanoro，它们表现出最高的酚酸含量。这些基因型代表了鹰嘴豆中抗氧化剂的良好来源，可用于改善其营养特性。
