School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China.
Bioresour Technol. 2018 Jun;257:281-289. doi: 10.1016/j.biortech.2018.02.118. Epub 2018 Feb 27.
The growing demand for biotechnological products against limited metabolic capacity of industrially used microorganisms has led to an increased interest on strain-improvement over the last several decades, which aimed to enhance metabolite yield, substrate uptake and tolerance of the strains. Among a few techniques of strain-improvement, genome shuffling is the most recent and promising approach used for rapid strain-improvement that can yield a new strain by combining whole genomes of multi-parental microorganisms using the principles of protoplast fusion. Genome shuffling has brought a major breakthrough in the strain-improvement concept as it is found to be effective and reliable for expressing complex phenotypes. This review will discuss the technical aspects and applications of genome shuffling for various industrial strains to present its current status and recent progress. In the concluding remarks, a summary will be presented focusing on the major challenges and future outlooks of this technology.
随着人们对生物技术产品的需求不断增长，而工业用微生物的代谢能力有限，过去几十年中，人们对菌株改良的兴趣日益浓厚，旨在提高代谢产物的产量、底物的吸收和菌株的耐受性。在几种菌株改良技术中，基因组改组是最近出现的、很有前途的快速菌株改良方法，它可以利用原生质体融合的原理，将多亲代微生物的全基因组组合在一起，从而产生新的菌株。基因组改组在菌株改良概念上带来了重大突破，因为它被发现对于表达复杂表型非常有效和可靠。本文将讨论基因组改组在各种工业菌株中的技术方面和应用，以展示其现状和最新进展。在结论部分，将重点介绍该技术的主要挑战和未来展望。
