College of Bioengineering/Collaborative Innovation Center of Grain Storage Security in Henan Province, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
World J Microbiol Biotechnol. 2020 Sep 24;36(10):158. doi: 10.1007/s11274-020-02936-w.
Genome shuffling, an efficient and practical strain improvement technology via recursive protoplasts fusion, can break through the limits of species even genus to accelerate the directed evolution of microbial strains, without requiring the comprehensively cognized genetic background and operable genetic system. Hence this technology has been widely used for many important strains to obtain the desirable industrial phenotypes. In this review, we introduce the procedure of genome shuffling, discuss the new aid strategies of genome shuffling, summarize the applications of genome shuffling for increasing metabolite yield, improving strain tolerance, enhancing substrate utilization, and put forward the outlook to the future development of this technology.
基因组改组，一种通过反复原生质体融合实现的高效实用的菌株改良技术，可以突破物种甚至属的限制，加速微生物菌株的定向进化，而无需全面了解遗传背景和可操作的遗传系统。因此，该技术已广泛应用于许多重要菌株，以获得理想的工业表型。在本文中，我们介绍了基因组改组的过程，讨论了基因组改组的新辅助策略，总结了基因组改组在提高代谢产物产量、提高菌株耐受性、增强底物利用等方面的应用，并对该技术的未来发展进行了展望。
