Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University,, Utrecht, The Netherlands.
Biomedical MR Imaging and Spectroscopy Group, Center for Image Sciences, University Medical Center Utrecht and Utrecht University, Utrecht, The Netherlands.
Pharm Res. 2018 Mar 8;35(4):88. doi: 10.1007/s11095-018-2348-y.
The aim of this study was to determine the potential of magnetic resonance imaging to evaluate the biodistribution of exogenous iron within 24 h after one single injection of Venofer® (iron sucrose).
Venofer® was evaluated in vitro for its ability to generate contrast in MR images. Subsequently, iron disposition was assessed in rats with MRI, in vivo up to 3 h and post mortem at 24 h after injection of Venofer®, at doses of 10- and 40 mg/kg body weight (n = 2 × 4), or saline (n = 4).
Within 10-20 min after injection of Venofer®, transverse relaxation rates (R) clearly increased, representative of a local increase in iron concentration, in liver, spleen and kidney, including the kidney medulla and cortex. In liver and spleen R values remained elevated up to 3 h post injection, while the initial R increase in the kidney was followed by gradual decrease towards baseline levels. Bone marrow and muscle tissue did not show significant increases in R values. Whole-body post mortem MRI showed most prominent iron accumulation in the liver and spleen at 24 h post injection, which corroborated the in vivo results.
MR imaging is a powerful imaging modality for non-invasive assessment of iron distribution in organs. It is recommended to use this whole-body imaging approach complementary to other techniques that allow quantification of iron disposition at a (sub)cellular level.
本研究旨在确定磁共振成像（MRI）在单次静脉注射蔗糖铁（Venofer®）后 24 小时内评估外源性铁在体内分布的潜力。
评估 Venofer®在 MRI 图像中产生对比的能力。随后，在大鼠中通过 MRI 评估铁的分布，在注射 Venofer®后 3 小时内和 24 小时时进行体内评估，剂量为 10-40mg/kg 体重（n=2×4），或盐水（n=4）。
在注射 Venofer®后 10-20 分钟内，肝脏、脾脏和肾脏（包括肾髓质和皮质）的横向弛豫率（R）明显增加，表明铁浓度局部增加。在肝和脾中，R 值在注射后 3 小时内保持升高，而在肾脏中，最初的 R 值增加后逐渐降至基线水平。骨髓和肌肉组织的 R 值没有明显增加。注射后 24 小时的全身 MRI 显示肝脏和脾脏中铁的积累最为明显，这与体内结果一致。
磁共振成像（MRI）是一种用于非侵入性评估器官中铁分布的强大成像方式。建议使用这种全身成像方法补充其他允许在（亚）细胞水平量化铁分布的技术。
