Bodjo Sanne Charles, Baziki Jean-de-Dieu, Nwankpa Nick, Chitsungo Ethel, Koffi Yao Mathurin, Couacy-Hymann Emmanuel, Diop Mariame, Gizaw Daniel, Tajelser Idris Badri Adam, Lelenta Mamadou, Diallo Adama, Tounkara Karim
African Union-Pan African Veterinary Vaccine Centre (AU-PANVAC), P. O. Box 1746, Debre Zeit, Ethiopia.
Laboratoire Central Veterinaire, B.P. 206, Bingerville, Côte d'Ivoire.
Arch Virol. 2018 Jul;163(7):1745-1756. doi: 10.1007/s00705-018-3782-1. Epub 2018 Mar 8.
Peste des petits ruminants (PPR) is a contagious and economically important disease affecting production of small ruminants (i.e., sheep and goats). Taking into consideration the lessons learnt from the Global Rinderpest Eradication Programme (GREP), PPR is now targeted by the international veterinary community as the next animal disease to be eradicated. To support the African continental programme for the control of PPR, the Pan African Veterinary Vaccine Centre of the African Union (AU-PANVAC) is developing diagnostics tools. Here, we describe the development of a blocking enzyme-linked immunosorbent assay (bELISA) that allows testing of a large number of samples for specific detection of antibodies directed against PPR virus in sheep and goat sera. The PPR bELISA uses an anti-haemagglutinin (H) monoclonal antibody (MAb) as a competitor antibody, and tests results are interpreted using the percentage of inhibition (PI) of MAb binding generated by the serum sample. PI values below or equal to 18% (PI ≤ 18%) are negative, PI values greater than or equal to 25% (PI ≥ 25%) are positive, and PI values greater than 18% and below 25% are doubtful. The diagnostic specificity (DSp) and diagnostic sensitivity (DSe) were found to be 100% and 93.74%, respectively. The H-based PPR-bELISA showed good correlation with the virus neutralization test (VNT), the gold standard test, with a kappa value of 0.947. The H-based PPR-bELISA is more specific than the commercial kit ID Screen® PPR Competition (N-based PPR-cELISA) from IDvet (France), but the commercial kit is slightly more sensitive than the H-based PPR-bELISA. The validation process also indicated good repeatability and reproducibility of the H-based PPR-bELISA, making this new test a suitable tool for the surveillance and sero-monitoring of the vaccination campaign.
小反刍兽疫（PPR）是一种具有传染性且对经济有重要影响的疾病，会影响小反刍动物（即绵羊和山羊）的生产。鉴于从全球牛瘟根除计划（GREP）中吸取的经验教训，国际兽医界目前将PPR作为下一个要根除的动物疾病。为支持非洲大陆控制PPR的计划，非洲联盟泛非兽医疫苗中心（AU-PANVAC）正在开发诊断工具。在此，我们描述了一种阻断酶联免疫吸附测定（bELISA）的开发，该方法可用于检测大量样本，以特异性检测绵羊和山羊血清中针对PPR病毒的抗体。PPR bELISA使用抗血凝素（H）单克隆抗体（MAb）作为竞争抗体，并使用血清样本产生的MAb结合抑制百分比（PI）来解释检测结果。PI值低于或等于18%（PI≤18%）为阴性，PI值大于或等于25%（PI≥25%）为阳性，PI值大于18%且低于25%为可疑。诊断特异性（DSp）和诊断敏感性（DSe）分别为100%和93.74%。基于H的PPR-bELISA与病毒中和试验（VNT，金标准试验）显示出良好的相关性，kappa值为0.947。基于H的PPR-bELISA比法国IDvet公司的商业试剂盒ID Screen® PPR Competition（基于N的PPR-cELISA）更具特异性，但该商业试剂盒的敏感性略高于基于H的PPR-bELISA。验证过程还表明基于H的PPR-bELISA具有良好的重复性和再现性，使这种新检测方法成为疫苗接种运动监测和血清监测的合适工具。
