Schopf R E, Hoffmann A, Jung M, Morsches B, Bork K
Arch Dermatol Res. 1986;279(2):89-94. doi: 10.1007/BF00417528.
Based on reports suggesting aberrant cell-mediated immunity and altered infiltration of immunocompetent cells into the skin in psoriasis, we studied the stimulation of T cells by autologous non-T mononuclear leukocytes (autologous mixed lymphocyte reaction, AMLR) and by epidermal cells isolated from lesional and clinically uninvolved skin in psoriasis (autologous mixed epidermal cell lymphocyte reaction, AMECLR). Age- and sex-matched individuals served as controls. We found that the AMLR in psoriasis (n = 11) was similar to that in healthy controls (n = 16); furthermore, cell proliferation was alike in the presence of either 5% AB-serum or autologous serum. By contrast, while the AMECLR in healthy controls (n = 9) resembled that in psoriatics employing epidermal cells from univolved skin, epidermal cells from lesional sites (n = 10) induced a significantly higher proliferation of autologous T cells in the AMECLR (P less than 0.01). We conclude that the in vitro stimulation of T cells by non-T mononuclear leukocytes is normal in psoriasis and is not regulated by autologous serum. Lesional psoriatic epidermal cells, however, are more active in stimulating autologous T cell proliferation than cells from univolved psoriatic or normal epidermis.
基于有报告提示银屑病中存在异常的细胞介导免疫以及免疫活性细胞向皮肤浸润的改变，我们研究了自体非T单核白细胞对T细胞的刺激作用（自体混合淋巴细胞反应，AMLR）以及从银屑病皮损处和临床未受累皮肤分离的表皮细胞对T细胞的刺激作用（自体混合表皮细胞淋巴细胞反应，AMECLR）。年龄和性别匹配的个体作为对照。我们发现，银屑病患者（n = 11）的AMLR与健康对照者（n = 16）相似；此外，在存在5% AB血清或自体血清的情况下，细胞增殖情况相同。相比之下，虽然健康对照者（n = 9）的AMECLR与使用未受累皮肤表皮细胞的银屑病患者相似，但皮损部位的表皮细胞（n = 10）在AMECLR中诱导自体T细胞增殖的能力明显更高（P小于0.01）。我们得出结论，在银屑病中，非T单核白细胞对T细胞的体外刺激作用正常，且不受自体血清调节。然而，银屑病皮损处的表皮细胞在刺激自体T细胞增殖方面比未受累银屑病或正常表皮的细胞更活跃。
