比较转录组分析揭示光照对小麦紫粒中花色苷生物合成的影响。

Comparative Transcriptome Analysis Revealing the Effect of Light on Anthocyanin Biosynthesis in Purple Grains of Wheat.

机构信息

State Key Laboratory of Crop Biology, College of Life Sciences , Shandong Agricultural University , Tai'an , 271018 Shandong , China.

College of Food Science and Engineering , Shandong Agricultural University , Tai'an , 271018 Shandong , China.

出版信息

J Agric Food Chem. 2018 Apr 4;66(13):3465-3476. doi: 10.1021/acs.jafc.7b05435. Epub 2018 Mar 21.

DOI:10.1021/acs.jafc.7b05435

PMID:29522338

Abstract

To study the mechanism of anthocyanin-biosynthesis regulation, we examined light-regulated gene expression involved in anthocyanin biosynthesis in purple grains of wheat. Ten kinds of anthocyanins were identified from a purple-grained wheat cultivar by HPLC-ESI-MS/MS analysis. Libraries constructed from the total RNA of purple grains under light (L) or dark (D) conditions for 15 or 20 days were sequenced. In total, 1874 differentially expressed genes (DEGs) were identified in L20 vs L15, 1432 DEGs were identified in D20 vs D15, 862 DEGs were identified in D15 vs L15, and 1786 DEGs were identified in D20 vs L20. DEG functional enrichments suggested that light-signal transduction is critical to anthocyanin biosynthesis. The 911 DEGs referred to as light-regulated DEGs (LDEGs) involved a number of genes in anthocyanin biosynthesis, transcription regulation, sugar- and calcium-signaling pathways, and hormone metabolism. These findings laid the foundation for future studies on the regulatory mechanisms of anthocyanin biosynthesis in purple grains of wheat.

摘要

为了研究花色苷生物合成调控的机制，我们研究了光调控花色苷生物合成相关基因在紫粒小麦中的表达。采用高效液相色谱-电喷雾串联质谱（HPLC-ESI-MS/MS）分析方法，从紫粒小麦品种中鉴定出 10 种花色苷。对紫粒小麦在光照（L）或黑暗（D）条件下分别处理 15 天和 20 天的总 RNA 构建的文库进行测序。结果表明，L20 与 L15 相比有 1874 个差异表达基因（DEGs），D20 与 D15 相比有 1432 个 DEGs，D15 与 L15 相比有 862 个 DEGs，D20 与 L20 相比有 1786 个 DEGs。DEG 功能富集分析表明，光信号转导对花色苷生物合成至关重要。这 911 个被称为光调控 DEGs（LDEGs）的 DEGs 涉及花色苷生物合成、转录调控、糖和钙信号通路以及激素代谢等多个基因。这些发现为今后研究紫粒小麦花色苷生物合成的调控机制奠定了基础。