Leduque P, Aratan-Spire S, Wolf B, Dubois P M, Czernichow P
Cell Tissue Res. 1987 Apr;248(1):89-94. doi: 10.1007/BF01239967.
Streptozotocin treatment at birth induces, in the pancreas of rats, first depletion of insulin and thyrotropin-releasing hormone and then early regeneration of beta cells and insulin, but not TRH. This study was undertaken to investigate whether the reduction in pancreatic TRH content can be associated with changes in the intensity and the distribution of TRH-immunoreactivity, and to follow the pattern of regeneration of beta cells through insulin- and TRH-immunoreactivity. In control animals, strong TRH-immunoreactivity was seen in insulin-containing cells on days 1-4 after birth. At day 7, the TRH-immunoreactivity was already decreased. In contrast, insulin-immunoreactivity was present throughout the neonatal period. A sparse population of cells near ducts also contained both TRH- and insulin-immunoreactivity at 1-2 days age. In streptozotocin-treated animals, TRH-immunoreactivity is found only in a few scattered insulin-containing cells in altered islets on days 1-4. Near the ducts, there were new insulin-containing cells which did not contain TRH. From day 7 regeneration of endocrine cells was characterized by new, typical islets, but these contained insulin- but not TRH-immunoreactivity. These findings suggest a differential control of the biosynthesis of insulin and TRH within the pancreas.
出生时用链脲佐菌素治疗可导致大鼠胰腺中胰岛素和促甲状腺激素释放激素（TRH）首先耗竭，随后β细胞和胰岛素早期再生，但TRH无再生。本研究旨在调查胰腺TRH含量的降低是否与TRH免疫反应性的强度和分布变化有关，并通过胰岛素和TRH免疫反应性追踪β细胞的再生模式。在对照动物中，出生后第1 - 4天在含胰岛素的细胞中可见强烈的TRH免疫反应性。在第7天，TRH免疫反应性已经降低。相比之下，胰岛素免疫反应性在整个新生儿期都存在。在1 - 2日龄时，导管附近少量细胞也同时含有TRH和胰岛素免疫反应性。在链脲佐菌素治疗的动物中，在第1 - 4天仅在改变的胰岛中少数散在的含胰岛素细胞中发现TRH免疫反应性。在导管附近，有新的含胰岛素细胞，但不含TRH。从第7天开始，内分泌细胞的再生以新的典型胰岛为特征，但这些胰岛含有胰岛素免疫反应性，而不含TRH免疫反应性。这些发现提示胰腺内胰岛素和TRH生物合成的差异调控。
