Kawano H, Daikoku S, Saito S
Endocrinology. 1983 Mar;112(3):951-5. doi: 10.1210/endo-112-3-951.
The location of TRH-like immunoreactivity (TRH-LI) was determined in developing rat pancreas (20.5 and 22.5 days of gestation, 1, 3, and 8 days, and 3 months after birth). The pancreatic tissue was freeze-dried and fixed in acrolein or paraformaldehyde vapor, and paraffin sections (2 microns) were prepared for immunostaining. TRH-LI was detected only in the islets. On day 20.5 of gestation, a few cells contained TRH-LI. During development, cells bearing TRH-LI increased in number and were most numerous in the early neonatal period (1-3 days). Then their number decreased again from 8 days after birth; in adult rats the immunoreactivity was too weak to allow identification of TRH-LI-positive cells. The topographical relation of TRH-LI with other islet hormones was studied in serial sections from perinatal rats stained with different antisera. TRH-LI was found in almost all cells containing insulin and in some containing glucagon, but not in those containing somatostatin.
在发育中的大鼠胰腺（妊娠20.5天和22.5天、出生后1天、3天、8天以及3个月）中确定了促甲状腺激素释放激素样免疫反应性（TRH-LI）的定位。胰腺组织经冷冻干燥后用丙烯醛或多聚甲醛蒸汽固定，制备2微米厚的石蜡切片用于免疫染色。仅在胰岛中检测到TRH-LI。在妊娠20.5天时，少数细胞含有TRH-LI。在发育过程中，带有TRH-LI的细胞数量增加，在新生儿早期（1 - 3天）数量最多。然后从出生后8天起其数量再次减少；在成年大鼠中，免疫反应性太弱，无法识别TRH-LI阳性细胞。在用不同抗血清染色的围产期大鼠连续切片中研究了TRH-LI与其他胰岛激素的拓扑关系。在几乎所有含胰岛素的细胞以及一些含胰高血糖素的细胞中发现了TRH-LI，但在含生长抑素的细胞中未发现。
