La Thangue N B, Rigby P W
Cell. 1987 May 22;49(4):507-13. doi: 10.1016/0092-8674(87)90453-3.
The expression of the E2A transcription unit of the adenovirus E1A deletion mutant dl312 in murine embryonal carcinoma (EC) stem cells suggests that they contain an activity that will complement viral E1A. We have prepared from these cells in vitro transcription extracts that use E1A-inducible promoters more efficiently than do extracts from a differentiated cell line. Mixing experiments demonstrate that the EC phenotype is dominant. Gel retardation assays using the E2A promoter detect a binding activity present in F9 and PCC4 EC cells but not in differentiated cells. Our data indicate that EC stem cells contain a transcription factor that is analogous to viral E1A and is likely to be involved in the control of cellular gene expression during differentiation.
腺病毒E1A缺失突变体dl312的E2A转录单位在小鼠胚胎癌（EC）干细胞中的表达表明，这些细胞含有一种能够互补病毒E1A的活性。我们从这些细胞制备了体外转录提取物，与来自分化细胞系的提取物相比，该提取物能更有效地利用E1A诱导型启动子。混合实验表明EC表型是显性的。使用E2A启动子的凝胶阻滞分析检测到F9和PCC4 EC细胞中存在一种结合活性，而在分化细胞中不存在。我们的数据表明，EC干细胞含有一种与病毒E1A类似的转录因子，可能参与分化过程中细胞基因表达的调控。
