玫瑰红孟加拉依附和葡聚糖涂层的氧化钆纳米颗粒用于潜在的诊断成像应用。
Rose Bengal attached and dextran coated gadolinium oxide nanoparticles for potential diagnostic imaging applications.
机构信息
Nanotechnology and Drug Delivery Research Lab, Department of Chemistry, University of Delhi, Delhi, India.
Nanotechnology and Drug Delivery Research Lab, Department of Chemistry, University of Delhi, Delhi, India; Institute of Nuclear Medicine and Allied Sciences, DRDO, Ministry of Defense, Delhi, India.
出版信息
Eur J Pharm Sci. 2018 May 30;117:362-370. doi: 10.1016/j.ejps.2018.03.008. Epub 2018 Mar 6.
We report here, reverse micelle mediated synthesis of multifunctional dextran (dex) coated GdO nanoparticles (NPs) carrying rose bengal (RB) dye for magnetic resonance and optical imaging. The diameter of these RB attached dex coated GdO NPs (Gd-dex-RB NPs) was found to be ~17 nm as measured by TEM. NMR line broadening effect on the surrounding water protons affirmed the paramagnetic nature of these NPs. Optical properties of Gd-dex-RB NPs were validated by UV-Vis and fluorescence spectroscopy. Time dependent release profile of RB from NPs at two different pH of 7.4 and 5.0 revealed that these NPs behave as slow releasing system. In-vitro study revealed that NPs are efficiently taken up by cells and show optical activity in cellular environment. In vitro cell viability (SRB) assay was performed on cancerous (A-549, U-87) and normal (HEK-293) cell lines, showed the absence of cytotoxic effect of Gd-dex-RB NPs. Therefore, such multifunctional NPs can be efficiently used for bio-imaging and optical tracking.
我们在此报告，反胶束介导的多功能葡聚糖（dex）包覆的 GdO 纳米颗粒（NPs）的合成，该纳米颗粒携带玫瑰红（RB）染料，用于磁共振和光学成像。通过 TEM 测量，这些 RB 连接的 dex 包覆的 GdO NPs（Gd-dex-RB NPs）的直径约为 17nm。对周围水质子的 NMR 线宽效应证实了这些 NPs 的顺磁性质。通过紫外可见光谱和荧光光谱验证了 Gd-dex-RB NPs 的光学性质。在两种不同 pH 值（7.4 和 5.0）下，从 NPs 中 RB 的时变释放曲线表明，这些 NPs 表现为缓慢释放系统。体外研究表明，NPs 被细胞有效摄取，并在细胞环境中表现出光学活性。在体外细胞活力（SRB）测定中，在癌细胞（A-549、U-87）和正常细胞（HEK-293）系上进行，结果表明 Gd-dex-RB NPs 没有细胞毒性作用。因此，这种多功能 NPs 可以有效地用于生物成像和光学跟踪。
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