Brune Nadine, Mues Benedikt, Buhl Eva Miriam, Hintzen Kai-Wolfgang, Jockenhoevel Stefan, Cornelissen Christian G, Slabu Ioana, Thiebes Anja Lena
Institute of Applied Medical Engineering, Helmholtz Institute, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany.
Institute of Pathology, Electron Microscopy Facility, University Clinic Aachen, 52074 Aachen, Germany.
Nanomaterials (Basel). 2023 Jun 16;13(12):1869. doi: 10.3390/nano13121869.
The interest in mesenchymal stromal cells as a therapy option is increasing rapidly. To improve their implementation, location, and distribution, the properties of these must be investigated. Therefore, cells can be labeled with nanoparticles as a dual contrast agent for fluorescence and magnetic resonance imaging (MRI). In this study, a more efficient protocol for an easy synthesis of rose bengal-dextran-coated gadolinium oxide (GdO-dex-RB) nanoparticles within only 4 h was established. Nanoparticles were characterized by zeta potential measurements, photometric measurements, fluorescence and transmission electron microscopy, and MRI. In vitro cell experiments with SK-MEL-28 and primary adipose-derived mesenchymal stromal cells (ASC), nanoparticle internalization, fluorescence and MRI properties, and cell proliferation were performed. The synthesis of GdO-dex-RB nanoparticles was successful, and they were proven to show adequate signaling in fluorescence microscopy and MRI. Nanoparticles were internalized into SK-MEL-28 and ASC via endocytosis. Labeled cells showed sufficient fluorescence and MRI signal. Labeling concentrations of up to 4 mM and 8 mM for ASC and SK-MEL-28, respectively, did not interfere with cell viability and proliferation. GdO-dex-RB nanoparticles are a feasible contrast agent to track cells via fluorescence microscopy and MRI. Fluorescence microscopy is a suitable method to track cells in in vitro experiments with smaller samples.
作为一种治疗选择，间充质基质细胞的关注度正在迅速增加。为了改进它们的应用、定位和分布，必须对这些细胞的特性进行研究。因此，可以用纳米颗粒作为荧光和磁共振成像（MRI）的双重造影剂对细胞进行标记。在本研究中，建立了一种更有效的方案，可在仅4小时内轻松合成孟加拉玫瑰红-葡聚糖包被的氧化钆（GdO-葡聚糖-RB）纳米颗粒。通过zeta电位测量、光度测量、荧光和透射电子显微镜以及MRI对纳米颗粒进行了表征。对SK-MEL-28和原代脂肪来源的间充质基质细胞（ASC）进行了体外细胞实验，检测了纳米颗粒的内化、荧光和MRI特性以及细胞增殖情况。GdO-葡聚糖-RB纳米颗粒的合成成功，并且在荧光显微镜和MRI中显示出足够的信号。纳米颗粒通过内吞作用内化到SK-MEL-28和ASC中。标记的细胞显示出足够的荧光和MRI信号。ASC和SK-MEL-28的标记浓度分别高达4 mM和8 mM时，均不影响细胞活力和增殖。GdO-葡聚糖-RB纳米颗粒是一种通过荧光显微镜和MRI追踪细胞的可行造影剂。荧光显微镜是在较小样本的体外实验中追踪细胞的合适方法。
