Department of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, People's Republic of China.
Department of Orthopaedics, Zibo Central Hospital, 54 West Gongqingtuan Road, Zibo, Shandong, People's Republic of China.
Calcif Tissue Int. 2018 Aug;103(2):227-236. doi: 10.1007/s00223-018-0410-8. Epub 2018 Mar 9.
As a regulator of osteogenesis, microRNA-218 (miR-218) is reported to promote osteogenesis of mesenchymal stem cells (MSCs). However, the in vivo osteogenic effect of miR-218 remains elusive. In this study, miR-218 was confirmed to promote osteogenic differentiation of MSCs by stimulating the alkaline phosphatase activity, calcium nodule formation, and osteogenic marker gene expression. For in vivo study, the miR-218-overexpressing BMSCs were locally administrated into the fracture sites in a femur fracture mouse model. Based on the X-rays, micro-computed tomography, mechanical testing, histology, and immunohistochemistry examinations, miR-218 overexpression improved new bone formation and accelerated fracture healing. These findings suggest that miR-218 may be a promising therapeutic target for bone repair in future clinical applications.
作为成骨的调节因子，microRNA-218（miR-218）据报道能促进间充质干细胞（MSCs）的成骨。然而，miR-218 的体内成骨作用仍不清楚。在这项研究中，miR-218 通过刺激碱性磷酸酶活性、钙结节形成和成骨标志物基因表达，被证实能促进 MSCs 的成骨分化。在体内研究中，miR-218 过表达的 BMSCs 被局部注射到股骨骨折小鼠模型的骨折部位。基于 X 射线、微计算机断层扫描、力学测试、组织学和免疫组织化学检查，miR-218 过表达促进了新骨形成并加速了骨折愈合。这些发现表明，miR-218 可能是未来临床应用中骨修复的有前途的治疗靶点。
