College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China.
School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
Int J Biol Macromol. 2018 Jul 1;113:881-888. doi: 10.1016/j.ijbiomac.2018.03.036. Epub 2018 Mar 8.
The pharaoh cuttlefish Sepia pharaonis is particularly sensitive to environmental changes in its breeding environment. The breeding of S. pharaonis larvae was carried out in different salinities for 48h, and the changes in survival rate, histological structure, energy metabolism, and anti-oxidative stress parameters were investigated and correlated with arginine kinase (AK) expression changes in muscle and liver tissues. The suitable salinity for larvae cultivation ranged from 24 to 30‰, and the survival rate showed a significant decline at 21‰ salinity. Histological observations of muscle and liver showed that changes in salinity and osmotic pressure had an adverse effect on tissue structure. Measurements of glycogen and lactic acid levels suggested that S. pharaonis could dynamically adjust energy metabolism to provide additional energy under unsuitable salinity. The protein levels and enzyme activities of AK in muscle significantly increased at 21‰ salinity. The results were consistent with prompt replenishment of phosphoarginine stores during salinity stress to maintain a dynamic ATP balance, suggesting that AK plays an important role in the regulation of energy metabolism. This study provides insight into metabolic changes during salinity stress and sheds light on the functional role of AK in S. pharaonis.
法老乌贼 Sepia pharaonis 对其繁殖环境中的环境变化特别敏感。将 S. pharaonis 幼虫在不同盐度下饲养 48h，研究存活率、组织学结构、能量代谢和抗氧化应激参数的变化，并与肌肉和肝脏组织中精氨酸激酶（AK）表达变化相关联。幼虫培养的适宜盐度范围为 24 至 30‰，盐度为 21‰时存活率显著下降。肌肉和肝脏的组织学观察表明，盐度和渗透压的变化对组织结构有不利影响。糖原和乳酸水平的测量表明，S. pharaonis 可以在不适宜的盐度下动态调节能量代谢，以提供额外的能量。21‰盐度下肌肉中 AK 的蛋白水平和酶活性显著增加。结果与在盐度胁迫期间迅速补充磷酸精氨酸储备以维持动态 ATP 平衡的结果一致，表明 AK 在能量代谢调节中起重要作用。本研究深入了解了盐度胁迫过程中的代谢变化，并揭示了 AK 在 S. pharaonis 中的功能作用。
