Matsuda M, Awata T, Ohashi Y, Inaba M, Fukuda M, Manabe R
Curr Eye Res. 1987 Feb;6(2):391-7. doi: 10.3109/02713688709025192.
Diabetic rats were produced by intravenous injection of streptozotocin. Of these, eleven rats were treated with topical instillation of 0.5% aldose reductase inhibitor (ARI), while ten received vehicle alone. The corneal endothelium of these diabetic rats was examined by specular microscopy and compared to age-matched nondiabetic rats (ten rats). Computerized morphometric analysis of individual cells demonstrated that the endothelium of the untreated diabetic rats had marked polymegathism (increased coefficient of variation in cell area) and pleomorphism (decreased percentage of hexagonal cells), as previously observed in diabetic patients. Similar endothelial changes were also noted in the ARI-treated diabetic rats, but to a significantly lesser extent. These results suggest that topically applied ARI can be effective in reducing morphologic changes of the diabetic endothelium, and that activation of the sorbitol pathway may be implicated in the etiology of such endothelial changes.
通过静脉注射链脲佐菌素制备糖尿病大鼠。其中，11只大鼠接受0.5%醛糖还原酶抑制剂（ARI）局部滴注治疗，而10只大鼠仅接受赋形剂。通过镜面显微镜检查这些糖尿病大鼠的角膜内皮，并与年龄匹配的非糖尿病大鼠（10只大鼠）进行比较。对单个细胞进行计算机形态计量分析表明，未经治疗的糖尿病大鼠的内皮有明显的大小不均（细胞面积变异系数增加）和多形性（六边形细胞百分比降低），正如先前在糖尿病患者中观察到的那样。在接受ARI治疗的糖尿病大鼠中也观察到类似的内皮变化，但程度明显较轻。这些结果表明，局部应用ARI可有效减少糖尿病内皮的形态学变化，并且山梨醇途径的激活可能与这种内皮变化的病因有关。
