Department of Animal Science, South Dakota State University, Brookings 57007.
Department of Animal Science, South Dakota State University, Brookings 57007.
J Dairy Sci. 2018 Jun;101(6):5159-5165. doi: 10.3168/jds.2017-13701. Epub 2018 Mar 7.
We conducted 2 experiments to determine lysine loss from 2 lipid-coated lysine products after mixing with silage. In our first experiment, we mixed 2 lipid-coated lysine products, crystalline lysine or crystalline lysine and amounts of lipid identical to amounts included in lipid-coated lysine products, with alfalfa or corn silage that had 2 different amounts of acidity. Lysine appeared to disassociate from lipid-coated lysine products in a nonlinear manner after mixing with either alfalfa or corn silage at different amounts of acidity. Additionally, silage source and acidity affected amounts of lysine released from lipid-coated lysine products after mixing. In a corresponding experiment, in vitro estimates of lysine available to ruminal microbiota after mixing with alfalfa or corn silage at different amounts of acidity were measured by ammonia release. In vitro measures were conducted with or without monensin to allow estimates of effects of monensin on amounts of lysine released from the 2 lipid-coated lysine products. It is unclear whether in vitro estimates of lysine fermentation from lipid-coated lysine are truly reflective of ruminal degradation of lysine from lipid-coated lysine because amounts of time needed to measure differences between different lysine sources were greater than typical estimates of mean ruminal particulate retention time. Nonetheless, monensin apparently reduced ammonia release from lysine, but ammonia release from lipid-coated lysine did not differ from crystalline lysine. Clearly, methods of manufacture together with physical and chemical characteristics of diet can affect amounts of lysine provided from lipid-coated lysine products to ruminants.
我们进行了两项实验,以确定在与青贮料混合后,两种脂质包被赖氨酸产品中的赖氨酸损失。在我们的第一项实验中,我们将两种脂质包被赖氨酸产品、结晶赖氨酸或与脂质包被赖氨酸产品中包含的量相同的结晶赖氨酸与具有两种不同酸度的苜蓿或玉米青贮料混合。赖氨酸在与不同酸度的苜蓿或玉米青贮料混合后,似乎以非线性方式从脂质包被赖氨酸产品中解离。此外,青贮料来源和酸度会影响混合后从脂质包被赖氨酸产品中释放的赖氨酸量。在相应的实验中,通过氨释放测量与不同酸度的苜蓿或玉米青贮料混合后微生物群可利用的赖氨酸的体外估计值。体外测量在有无莫能菌素的情况下进行,以允许估计莫能菌素对从两种脂质包被赖氨酸产品中释放的赖氨酸量的影响。尚不清楚从脂质包被赖氨酸中体外估计赖氨酸发酵是否真正反映了脂质包被赖氨酸在瘤胃中的降解,因为测量不同赖氨酸源之间差异所需的时间量大于典型的瘤胃颗粒滞留时间估计值。尽管如此,莫能菌素显然减少了赖氨酸的氨释放,但脂质包被赖氨酸的氨释放与结晶赖氨酸没有差异。显然,制造方法以及日粮的物理和化学特性会影响从脂质包被赖氨酸产品向反刍动物提供的赖氨酸量。