School of Environment Science and Spatial Informatics, China University of Mining and Technology, No.1, Daxue Road, Xuzhou, Jiangsu, 221116, China.
College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong, 510642, China.
Environ Sci Pollut Res Int. 2018 May;25(15):14437-14446. doi: 10.1007/s11356-018-1604-3. Epub 2018 Mar 10.
This study evaluated the effects of two surfactants (i.e., Tween 80 and SDS) on biodegradation of crude oil by mixed bacterial consortium in soil-aqueous system. The mixed bacterial consortium was domesticated from the activated sludge of cooking plant through a progressive domestication process. High-throughput sequencing analysis revealed that Rhodanobacter sp. was the dominant bacteria. The higher CMC value for two surfactants was observed in soil-aqueous system compared with that in aqueous system, which was likely due to their adsorption onto soil particles. Either Tween 80 or SDS can be utilized as carbon source and promote the growth of mixed bacterial consortium. Further findings evidenced that the degradation of crude oil can be enhanced by adding either Tween 80 or SDS. The performance of Tween 80 was generally superior to SDS for the crude oil degradation. The highest crude oil degradation efficiency was 42.2 and 31.0% under the conditions of 5 CMC of Tween 80 and 2 CMC of SDS, respectively. Furthermore, the degradation efficiency of crude oil in remediation experiment (i.e., 77%) evidenced that the integration of adding Tween 80 and inoculating mixed bacterial consortium was effective for crude oil-contaminated soil decontamination.
本研究评估了两种表面活性剂（即吐温 80 和 SDS）在土壤-水体系中混合细菌群落对原油生物降解的影响。混合细菌群落是通过渐进驯化过程从烹饪厂的活性污泥中驯化而来的。高通量测序分析表明，Rhodanobacter sp.是优势细菌。与在水溶液中相比，两种表面活性剂在土壤-水溶液中的 CMC 值更高，这可能是由于它们吸附在土壤颗粒上。吐温 80 或 SDS 都可以用作碳源并促进混合细菌群落的生长。进一步的研究结果表明，添加吐温 80 或 SDS 均可增强原油的降解。添加吐温 80 的效果通常优于 SDS。在 5 CMC 吐温 80 和 2 CMC SDS 的条件下，原油的降解效率最高分别为 42.2%和 31.0%。此外，修复实验中的原油降解效率（即 77%）表明，添加吐温 80 和接种混合细菌群落的整合对于受原油污染的土壤的去污是有效的。
