Sroka Jolanta, Zimolag Eliza, Lasota Slawomir, Korohoda Wlodzimierz, Madeja Zbigniew
Faculty of Biochemistry, Biophysics and Biotechnology, Department of Cell Biology, Jagiellonian University, Krakow, Poland.
Methods Mol Biol. 2018;1749:325-340. doi: 10.1007/978-1-4939-7701-7_23.
Electrotaxis plays an important role during embryogenesis, inflammation, wound healing, and tumour metastasis. However, the mechanisms at play during electrotaxis are still poorly understood. Therefore intensive studies on signaling pathways involved in this phenomenon should be carried out. In this chapter, we described an experimental system for studying electrotaxis of Amoeba proteus, mouse embryonic fibroblasts (MEF), Walker carcinosarcoma cells WC256, and bone marrow adherent cells (BMAC).
电趋性在胚胎发育、炎症、伤口愈合和肿瘤转移过程中发挥着重要作用。然而，电趋性过程中起作用的机制仍知之甚少。因此，应该对参与这一现象的信号通路进行深入研究。在本章中，我们描述了一个用于研究变形虫、小鼠胚胎成纤维细胞（MEF）、沃克癌肉瘤细胞WC256和骨髓贴壁细胞（BMAC）电趋性的实验系统。
