Cohen J H, Aubry J P, Jouvin M H, Wijdenes J, Bancherau J, Kazatchkine M, Revillard J P
J Immunol Methods. 1987 May 4;99(1):53-8. doi: 10.1016/0022-1759(87)90031-7.
A sensitive enhancing system was developed for detecting low density cell surface antigen by flow cytometry. The system termed the 'super avidin-biotin system' (SABS) uses biotinylated antibody, phycoerythrin-streptavidin (StreptA-PE), biotinylated goat anti-streptavidin antibody, and StreptA-PE. CR1 complement receptor antigenic sites were quantified on erythrocytes from healthy individuals and patients with antibodies against human immunodeficiency virus (HIV) using SABS and a conventional radioimmunoassay (RIA) with monoclonal anti-CR1 antibody. As little as 50 sites/cell were detected using either SABS or RIA. Accurate quantification of CR1 antigenic sites was achieved within the range of 100-1300 sites/cell. Similar results were obtained using either of the two methods. Intra-assay and day-to-day reproducibilities using SABS were 2% and 12% respectively, comparable to those of conventional RIA measurements. In addition to enumeration of CR1 on erythrocytes for clinical purposes, the use of SABS may probably be extended to a wide number of situations where a sensitive detection of low density cell surface antigen is needed.
开发了一种用于通过流式细胞术检测低密度细胞表面抗原的灵敏增强系统。该系统称为“超级抗生物素蛋白-生物素系统”（SABS），使用生物素化抗体、藻红蛋白-链霉抗生物素蛋白（StreptA-PE）、生物素化山羊抗链霉抗生物素蛋白抗体和StreptA-PE。使用SABS和具有单克隆抗CR1抗体的传统放射免疫测定（RIA），对健康个体和抗人类免疫缺陷病毒（HIV）抗体患者的红细胞上的CR1补体受体抗原位点进行了定量。使用SABS或RIA检测到低至每个细胞50个位点。在每个细胞100 - 1300个位点的范围内实现了CR1抗原位点的准确定量。使用这两种方法中的任何一种都获得了相似的结果。使用SABS的批内和日间重现性分别为2%和12%，与传统RIA测量的重现性相当。除了出于临床目的对红细胞上的CR1进行计数外，SABS的应用可能还会扩展到需要灵敏检测低密度细胞表面抗原的多种情况。
